Difference between revisions of "Part:BBa K3262002"

 
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<partinfo>BBa_K3262002 parameters</partinfo>
 
<partinfo>BBa_K3262002 parameters</partinfo>
 
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== Overlap Extension PCR to develop construct (2019 SNU_India) ==
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<img style="vertical-align: bottom;)" width=60% src="https://parts.igem.org/File:T--SNU_India--OPCR1.png ">
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Figure 1:Agarose gel electrophoresis of IDT gBlocks suspended in milliQ and loaded on 1% agarose gel.
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As seen in the gels the 1.4 kb NT-ESRP gBlock fragment showed band of expected size, however, 2.1 kb CT_ESRP gBlock had contaminating band at around 1.5kb along with the expected band of 2.1 kb. These fragments were then PCR-amplified with primers containing overlap overhang sequences for joining the fragments (Figure 2). However, the 2.1 kb fragment always gave unwanted amplification of a 1.5 kb fragment which also contaminated the original gBlock product.
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Revision as of 01:23, 22 October 2019


J23115+Estrogen-SensitiveT7RNAP

Estrogen sensitive T7 RNA polymerase wit constitutive promoter

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NotI site found at 2577
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 819
    Illegal XhoI site found at 800
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2315
    Illegal AgeI site found at 1418
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1145
    Illegal BsaI.rc site found at 2606
    Illegal SapI.rc site found at 560


Overlap Extension PCR to develop construct (2019 SNU_India)

Figure 1:Agarose gel electrophoresis of IDT gBlocks suspended in milliQ and loaded on 1% agarose gel.