Difference between revisions of "Part:BBa I712004:Experience"
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===User Reviews=== | ===User Reviews=== | ||
| − | We validated this part by insert it and eGFP together into the vertor. The map of the plasmid is as followed(Fig.1). Then we transfected the plasmid to 293T cells, the expression of eGFP protein was observed by fluorescence microscope(Fig.2). You can also see the characterization of this part in our composite part BBa_K2942705, BBa_K2942706, BBa_K2942707, BBa_K2942708. | + | ===edit by sysu-medicine=== |
| − | [[ | + | We validated this part by insert it and eGFP together into the vertor. The map of the plasmid is as followed(Fig.1). Then we transfected the plasmid to 293T cells, and the expression of eGFP protein was observed by fluorescence microscope(Fig.2) and quantified by Image J(Fig.3). You can also see the characterization of this part in our composite part BBa_K2942705, BBa_K2942706, BBa_K2942707, BBa_K2942708. |
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| + | [[file:lyt911.jpg|720px]] | ||
Fig.1 The plasmid we used for transfection. | Fig.1 The plasmid we used for transfection. | ||
| − | Fig.2 Transfection cells observed under a fluorescence microscope | + | |
| + | [[file:lyt912.jpg|720px]] | ||
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| + | Fig.2 Transfection cells observed under a fluorescence microscope. | ||
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| + | [[file:lyt043.jpg|720px]] | ||
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| + | Fig.3 Fluorescent quantitation by Image J(P<0.001). | ||
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Latest revision as of 01:22, 22 October 2019
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_I712004
Usage and Biology
User Reviews
edit by sysu-medicine
We validated this part by insert it and eGFP together into the vertor. The map of the plasmid is as followed(Fig.1). Then we transfected the plasmid to 293T cells, and the expression of eGFP protein was observed by fluorescence microscope(Fig.2) and quantified by Image J(Fig.3). You can also see the characterization of this part in our composite part BBa_K2942705, BBa_K2942706, BBa_K2942707, BBa_K2942708.
Fig.1 The plasmid we used for transfection.
Fig.2 Transfection cells observed under a fluorescence microscope.
Fig.3 Fluorescent quantitation by Image J(P<0.001).
UNIQ21175b7b398eab11-partinfo-00000000-QINU UNIQ21175b7b398eab11-partinfo-00000001-QINU