Difference between revisions of "Part:BBa I712004:Experience"
 
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===Applications of BBa_I712004===
 
===Applications of BBa_I712004===
 
===Usage and Biology===
 
===Usage and Biology===
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We validated this part by insert it and eGFP together into the vertor. The map of the plasmid is as followed(Fig.1). Then we transfected the plasmid to 293T cells, the expression of eGFP protein was observed by fluorescence microscope(Fig.2). You can also see the characterization of this part in our composite part BBa_K2942705, BBa_K2942706, BBa_K2942707, BBa_K2942708.
 
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[[File:2004_figure1.jpg]]Fig.1 The plasmid we used for transfection.
 
  
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Fig.2 Transfection cells observed under a fluorescence microscope
 
 
===User Reviews===
 
===User Reviews===
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===edit by sysu-medicine===
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We validated this part by insert it and eGFP together into the vertor. The map of the plasmid is as followed(Fig.1). Then we transfected the plasmid to 293T cells, and the expression of eGFP protein was observed by fluorescence microscope(Fig.2) and quantified by Image J(Fig.3). You can also see the characterization of this part in our composite part BBa_K2942705, BBa_K2942706, BBa_K2942707, BBa_K2942708.
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[[file:lyt911.jpg|720px]]
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Fig.1 The plasmid we used for transfection.
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[[file:lyt912.jpg|720px]]
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Fig.2 Transfection cells observed under a fluorescence microscope.
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[[file:lyt043.jpg|720px]]
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Fig.3 Fluorescent quantitation by Image J(P<0.001).
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Latest revision as of 01:22, 22 October 2019


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_I712004

Usage and Biology

User Reviews

edit by sysu-medicine

We validated this part by insert it and eGFP together into the vertor. The map of the plasmid is as followed(Fig.1). Then we transfected the plasmid to 293T cells, and the expression of eGFP protein was observed by fluorescence microscope(Fig.2) and quantified by Image J(Fig.3). You can also see the characterization of this part in our composite part BBa_K2942705, BBa_K2942706, BBa_K2942707, BBa_K2942708.


Lyt911.jpg

Fig.1 The plasmid we used for transfection.


Lyt912.jpg

Fig.2 Transfection cells observed under a fluorescence microscope.


Lyt043.jpg

Fig.3 Fluorescent quantitation by Image J(P<0.001).

UNIQ171dae46a21aa956-partinfo-00000000-QINU UNIQ171dae46a21aa956-partinfo-00000001-QINU