Difference between revisions of "Part:BBa K117004:Experience"

 
 
(4 intermediate revisions by one other user not shown)
Line 1: Line 1:
  
−
__NOTOC__
+
=='''Abnormality found in the registry'''==
−
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
+
This device could be found in the registry in the form of [https://parts.igem.org/wiki/index.php?title=Part:BBa_J04430 BBa_J04430]. However, the team found an abnormality when BBa_J04430 was transformed into Top 10 cells. As shown on the pictures below, the cells glow pink instead of green under UV light.
−
how you used this part and how it worked out.
+
  
−
===Applications of BBa_K117004===
+
[[image:NTU@iGEM_24062008_(pink_gfp).JPG|thumb|center|300px| BBa_J04430 glows pink]]
 +
 
 +
 
 +
=='''Characterization of pLacI-GFP'''==
 +
The NTU@iGEM team characterized the biobrick part pLacI-GFP. The influence of 1)concentration of Lactose 2) temperature and 3) time were investigated.
 +
 
 +
[[image:NTU@iGEM_Diagram2_of_characterization_pLacI-GFP.JPG|thumb|center|400px| The characterization overlook]]
 +
Shown below is the graph obtained for the experiment conducted at 37°C.
 +
[[image:NTU@iGEM_37norm.jpg|thumb|center|400px| Characterization at 37°C]]
 +
 
 +
===Electron microscope pictures===
 +
[[image:NTU@iGEM_Fluorscence_LacI-GFP_1mM.JPG|thumb|center|300px| Cells induced with 1mM lactose concentration]]
 +
[[image:NTU@iGEM_Fluorscence_LacI-GFP_10mM.JPG|thumb|center|300px| Cells induced with 10mM lactose concentration]]
 +
 
 +
 
 +
For more information including the protocols and results obtained, please go to:
 +
[http://2008.igem.org/Team:NTU-Singapore/Parts/Characterization_of_LacI-GFP NTU@iGEM Characterization of pLac-GFP]
  
 
===User Reviews===
 
===User Reviews===

Latest revision as of 01:01, 27 October 2008

Abnormality found in the registry

This device could be found in the registry in the form of BBa_J04430. However, the team found an abnormality when BBa_J04430 was transformed into Top 10 cells. As shown on the pictures below, the cells glow pink instead of green under UV light.

BBa_J04430 glows pink


Characterization of pLacI-GFP

The NTU@iGEM team characterized the biobrick part pLacI-GFP. The influence of 1)concentration of Lactose 2) temperature and 3) time were investigated.

The characterization overlook

Shown below is the graph obtained for the experiment conducted at 37°C.

Characterization at 37°C

Electron microscope pictures

Cells induced with 1mM lactose concentration
Cells induced with 10mM lactose concentration


For more information including the protocols and results obtained, please go to: [http://2008.igem.org/Team:NTU-Singapore/Parts/Characterization_of_LacI-GFP NTU@iGEM Characterization of pLac-GFP]

User Reviews

UNIQ43b75fcb8bb6dd6f-partinfo-00000000-QINU UNIQ43b75fcb8bb6dd6f-partinfo-00000001-QINU