Difference between revisions of "Part:BBa K2950027"
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In our study, we aim to find the equation of the expression level of the protein todD and the IPTG concentration. Therefore, we use GFP which is downstream the open reading frame todD as an indicator by measuring its fluorescent strength at different IPTG concentration to find the equation to determine their relationship. | In our study, we aim to find the equation of the expression level of the protein todD and the IPTG concentration. Therefore, we use GFP which is downstream the open reading frame todD as an indicator by measuring its fluorescent strength at different IPTG concentration to find the equation to determine their relationship. | ||
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| + | <img src="https://static.igem.org/mediawiki/parts/5/50/T--BNDS_China--todD-GFP.png" width=600px></br> | ||
| + | <p style="width:600px; margin-left:150px; margin-bottom:60px; | ||
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| + | "><em><strong>FIGURE 1</strong>The graph about the intensity of fluorescence per OD and IPTG concentration</em> </p> | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 01:15, 22 October 2019
lac operator-todD-GFP
In our study, we aim to find the equation of the expression level of the protein todD and the IPTG concentration. Therefore, we use GFP which is downstream the open reading frame todD as an indicator by measuring its fluorescent strength at different IPTG concentration to find the equation to determine their relationship.
FIGURE 1The graph about the intensity of fluorescence per OD and IPTG concentration
Sequence and Features