Difference between revisions of "Part:BBa I712004:Experience"
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Fig.1 The plasmid we used for transfection.
 
Fig.1 The plasmid we used for transfection.
  

Revision as of 01:11, 22 October 2019


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Applications of BBa_I712004

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edit by sysu-medicine

We validated this part by insert it and eGFP together into the vertor. The map of the plasmid is as followed(Fig.1). Then we transfected the plasmid to 293T cells, and the expression of eGFP protein was observed by fluorescence microscope(Fig.2) and quantified by Image J(Fig.3). You can also see the characterization of this part in our composite part BBa_K2942705, BBa_K2942706, BBa_K2942707, BBa_K2942708.


Lyt911.jpg

Fig.1 The plasmid we used for transfection.


Lyt042.jpg

Fig.2 Transfection cells observed under a fluorescence microscope.


Lyt043.jpg

Fig.3 Fluorescent quantitation by Image J(P<0.001).

UNIQ10115806c6018b4b-partinfo-00000000-QINU UNIQ10115806c6018b4b-partinfo-00000001-QINU