Difference between revisions of "Part:BBa K3042000"
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<partinfo>BBa_K3042000 short</partinfo>
 
<partinfo>BBa_K3042000 short</partinfo>
  
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This part is a codon-optimized version of RFP for use in use in Dinoflagellates, a single-celled organism. This part was created to be used in DinoIII, a dinoflagellate backbone vector.  The sequence for this part was synthesized via Snapgene. Considering dinoflagellates have many codons that code for the same amino acids, favorable codons of the dinoflagellates were chosen using the chart below to ensure the translation of the red fluorescent protein. This part can be used as a reporter or indicator of a successful transformation. Positive results are demonstrated by red fluorescence emitted from the cells.
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<p>This part is a codon optimized version of RFP for use in use in Dinoflagellates, a single celled organism. This part was created to be used in DinoIII, a dinoflagellate backbone vector .  The sequence for this part was synthesized via Snapgene. Considering dinoflagellates have preference when it comes to specific  amino acids, the favorable codons of the dinoflagellates were chosen using the chart below to ensure the successful translation of the red fluorescent protein. This part can be used as a reporter/indicator of a successful transformation. Positive results are demonstrated by red fluorescence emitted from the cells.<p>
  
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[Insert chart picture here]
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<img src="https://2019.igem.org/wiki/images/e/e9/T--Georgia_State--codonchart.png"></img>
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<p>Figure 1. Codon Optimized Chart for Oxyrrhis marina<p>
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<p>This was the chart we used to design the RFP part in Snapgene, to be edited into the Dino III plasmid in place of the existing GFP part BBa_K3042005<p>
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<p>mRFP1- monomeric derivative of Dsred (Campbell et al., 2002)<p>
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<p>Brittany N. Sprecher, Huan Zhang, Senjie Lin (2019, April 9). Nuclear gene transformation in a dinoflagellate. doi: 10.1101/602821<p>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 00:54, 22 October 2019


Dinoflagellate Codon Optimized RFP

This part is a codon optimized version of RFP for use in use in Dinoflagellates, a single celled organism. This part was created to be used in DinoIII, a dinoflagellate backbone vector . The sequence for this part was synthesized via Snapgene. Considering dinoflagellates have preference when it comes to specific amino acids, the favorable codons of the dinoflagellates were chosen using the chart below to ensure the successful translation of the red fluorescent protein. This part can be used as a reporter/indicator of a successful transformation. Positive results are demonstrated by red fluorescence emitted from the cells.<p> <img src="T--Georgia_State--codonchart.png"></img> <p>Figure 1. Codon Optimized Chart for Oxyrrhis marina<p> <p>This was the chart we used to design the RFP part in Snapgene, to be edited into the Dino III plasmid in place of the existing GFP part BBa_K3042005<p> <p>mRFP1- monomeric derivative of Dsred (Campbell et al., 2002)<p> <p>Brittany N. Sprecher, Huan Zhang, Senjie Lin (2019, April 9). Nuclear gene transformation in a dinoflagellate. doi: 10.1101/602821<p> Sequence and Features

Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 13
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 696
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 13
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 13
  • 1000
    COMPATIBLE WITH RFC[1000]