Difference between revisions of "Part:BBa K3156888"
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<p style="text-align:center;"><img src="https://2019.igem.org/wiki/images/0/06/T--SHSBNU_China---dual_repair.png" width = "700" height ="500"/> | <p style="text-align:center;"><img src="https://2019.igem.org/wiki/images/0/06/T--SHSBNU_China---dual_repair.png" width = "700" height ="500"/> | ||
<figcaption></p> | <figcaption></p> | ||
− | <p style="text-align:center;"><b>Figure 1.Repairing result of BBa_K3156888(<i>sfgfp</i> | + | <p style="text-align:center;"><b>Figure 1.Repairing result of BBa_K3156888(<i>sfgfp</i> forward).</b> </p> |
</figcaption> | </figcaption> |
Revision as of 00:47, 22 October 2019
pLac Promoter-ssDNA[sfGFP(ON)]-Ec86-Beta
Design
We combined msd-msr cassette, Reverse Transcriptase Ec-86 and Beta recombinase together to create BBa_K3156888. Msd-msr cassette can produce mRNA for reverse transcription to produce
We created a
Usage and Biology
Reference
[1]F. Farzadfard, T. K. Lu, Science 346,1256272 (2014). DOI: 10.1126/science.1256272
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 9
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1
Illegal XhoI site found at 516 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]