Difference between revisions of "Part:BBa K2010999"
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Revision as of 00:30, 22 October 2019
PETase (PET-degrading enzyme, origin I. sakaiensis)
PETase is a poly(ethylene terehphthalate)-degrading enzyme first identified in Ideonella sakaiensis. This sequence is the E. coli K12 optimized DNA sequence for PETase.
https://parts.igem.org/File:T--Toronto--pNPB-Hydrolysis-pH-8-Wildtype-All-Conc.png https://parts.igem.org/File:T--Toronto--pNPB-Hydrolysis-pH-9_Wildtype-All-Conc.png https://parts.igem.org/File:T--Toronto--pNPB-Hydrolysis-Wildtype_pH7-8-9.png
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 67
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]