Difference between revisions of "Part:BBa K3002110"

 
Line 2: Line 2:
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K3002110 short</partinfo>
 
<partinfo>BBa_K3002110 short</partinfo>
−
 
+
<html>
−
This composite part contains the PAR-promoter (BBa_K3002027) in combination with the RPL23-Terminator (BBa_K3002006), GLE secretion signal (BBa_K3002008), SP20 HA-tag (BBa_K3002010) and the coding sequence of the mutant PETase (BBa_K3002014) plus the MoClo connectors for positions B1-B2 (BBa_K3002302), B2-B3 (BBa_K3002303), B4-B5 (BBa_K3002304) and B5-B6 (BBa_K3002305).  
+
This composite part contains the PAR-promoter (<a href="https://parts.igem.org/Part:BBa_K3002027">BBa_K3002027</a>) in combination with the RPL23-Terminator (<a href="https://parts.igem.org/Part:BBa_K3002006">BBa_K3002006</a>), GLE secretion signal (<a href="https://parts.igem.org/Part:BBa_K3002008">BBa_K3002008</a>), SP20 HA-tag (<a href="https://parts.igem.org/Part:BBa_K3002010">BBa_K3002010</a>) and the coding sequence of the mutant PETase (<a href="https://parts.igem.org/Part:BBa_K3002014">BBa_K3002014</a>) plus the MoClo connectors for positions B1-B2 (<a href="https://parts.igem.org/Part:BBa_K3002302">BBa_K3002302</a>), B2-B3 (<a href="https://parts.igem.org/Part:BBa_K3002303">BBa_K3002303</a>), B4-B5 (<a href="https://parts.igem.org/Part:BBa_K3002304">BBa_K3002304</a>) and B5-B6 (<a href="https://parts.igem.org/Part:BBa_K3002305">BBa_K3002305</a>).  
−
 
+
</html>
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 00:26, 22 October 2019


Level 1 - Mutant PETase + GLE + SP20HA (Phytobrick) This composite part contains the PAR-promoter (BBa_K3002027) in combination with the RPL23-Terminator (BBa_K3002006), GLE secretion signal (BBa_K3002008), SP20 HA-tag (BBa_K3002010) and the coding sequence of the mutant PETase (BBa_K3002014) plus the MoClo connectors for positions B1-B2 (BBa_K3002302), B2-B3 (BBa_K3002303), B4-B5 (BBa_K3002304) and B5-B6 (BBa_K3002305). Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1087
    Illegal PstI site found at 2060
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 249
    Illegal PstI site found at 1087
    Illegal PstI site found at 2060
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1087
    Illegal PstI site found at 2060
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1087
    Illegal PstI site found at 2060
    Illegal NgoMIV site found at 822
    Illegal NgoMIV site found at 849
    Illegal NgoMIV site found at 2620
  • 1000
    COMPATIBLE WITH RFC[1000]