Difference between revisions of "Part:BBa K819010:Experience"

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<h1>characterizon of BBa_K819010 in CL1(the ''cheZ'' lacking strain of ''Escherichia coli'')</h1>.
 
<h1>characterizon of BBa_K819010 in CL1(the ''cheZ'' lacking strain of ''Escherichia coli'')</h1>.
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We used an ''cheZ'' lacking strain of ''E. coli'' K12 MG1655  
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<img src="https://static.igem.org/mediawiki/parts/c/c6/T--HZAU--China--CL1_with_K819010.png" style="width:500px;margin-right:20%;"/>
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We used an ''cheZ'' lacking strain of ''E. coli'' K12 MG1655 transformed with this kit, BBa_K819010, to process swarming assay.
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The result is shown in the figure above. We measured the clony's radius of CL1 and CL1 transformed with BBa_K819010 at 17 hours, 36 hours and 48 hours separately. The result shows significant function of BBa_K819010, but motility of the strain transformed with BBa_K819010 is still weaker than  that of MG1655, wild type (data not shown).
 
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<partinfo>BBa_K819010 AddReview 5</partinfo>
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<I>2019 iGEM Michigan State</I>
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<h1>Characterizon of the burden of BBa_K819010 in E. coli JEB1203 Burden Monitor Strain</h1>.
 
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<img src="https://static.igem.org/mediawiki/parts/c/c6/T--HZAU--China--CL1_with_K819010.png" style="width:500px;margin-left:5%;"/>
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Using a strain that UT Austin iGEM team made, JEB1203, we can measure the burden of a a plasmid. This works because JEB1203 has GFP in the chromosome and when a plasmid that has a high burden, or takes a lot of energy from chromosomal replication/transcription, the GFP will not fluoresce as much. However, since most parts added are not necessary to the bacteria the bacteria will get rid of the part or cause mutation to lighten that burden. From this information we can tell whether or not a part has a high burden on the bacteria or not. This is how we decided the burden of part BBa_K819010.
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<img src="https://static.igem.org/mediawiki/parts/b/b2/JEB1203_MSU_Growth_Curve.png" style="width:500px;margin-right:20%;"/>
 
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We used the JEB1203 Burden monitor strain that has GFP in the chromosome to show how much of a burden part has on a cell. The higher the burden of a part means that the GFP and OD will be lower because the part is taking up more energy away from chromosomal replication and expression. This graph shows the expected OD and GFP levels of the JEB1203 E. coli strain with no burden.
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<img src="https://static.igem.org/mediawiki/parts/5/5b/JEB1206_MSU_Growthcurve.png" style="width:500px;margin-right:20%;"/>
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This graph shows the OD and GFP fluorescence of the JEB1206 burden monitor E. coli strain, which has a medium/high burden. To be noted in this graph is the lower ODs and GFP levels than that of the monitor strain JEB1203, which has no burden. 
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<img src="https://static.igem.org/mediawiki/parts/3/3b/K819010_MSU_Growthcurve.png" style="width:500px;margin-right:20%;"/>
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This graph is the BBa_K819010 part that codes for cheZ transformed into the JEB1203 burden monitor E. coli strain. From this graph it can be seen that the OD and GFP levels are at very similar levels of the JEB1203 strain that did not have any burden. From this data we characterized the burden of BBa_K819010 as little to none.
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Latest revision as of 00:21, 22 October 2019


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K819010

User Reviews

UNIQ3e2518ba16223a18-partinfo-00000000-QINU

•••••

2016iGEM_HZAU

characterizon of BBa_K819010 in CL1(the cheZ lacking strain of Escherichia coli)

.

We used an cheZ lacking strain of E. coli K12 MG1655 transformed with this kit, BBa_K819010, to process swarming assay. The result is shown in the figure above. We measured the clony's radius of CL1 and CL1 transformed with BBa_K819010 at 17 hours, 36 hours and 48 hours separately. The result shows significant function of BBa_K819010, but motility of the strain transformed with BBa_K819010 is still weaker than that of MG1655, wild type (data not shown).

;


•••••

2019 iGEM Michigan State

Characterizon of the burden of BBa_K819010 in E. coli JEB1203 Burden Monitor Strain

.

Using a strain that UT Austin iGEM team made, JEB1203, we can measure the burden of a a plasmid. This works because JEB1203 has GFP in the chromosome and when a plasmid that has a high burden, or takes a lot of energy from chromosomal replication/transcription, the GFP will not fluoresce as much. However, since most parts added are not necessary to the bacteria the bacteria will get rid of the part or cause mutation to lighten that burden. From this information we can tell whether or not a part has a high burden on the bacteria or not. This is how we decided the burden of part BBa_K819010. We used the JEB1203 Burden monitor strain that has GFP in the chromosome to show how much of a burden part has on a cell. The higher the burden of a part means that the GFP and OD will be lower because the part is taking up more energy away from chromosomal replication and expression. This graph shows the expected OD and GFP levels of the JEB1203 E. coli strain with no burden.

This graph shows the OD and GFP fluorescence of the JEB1206 burden monitor E. coli strain, which has a medium/high burden. To be noted in this graph is the lower ODs and GFP levels than that of the monitor strain JEB1203, which has no burden. This graph is the BBa_K819010 part that codes for cheZ transformed into the JEB1203 burden monitor E. coli strain. From this graph it can be seen that the OD and GFP levels are at very similar levels of the JEB1203 strain that did not have any burden. From this data we characterized the burden of BBa_K819010 as little to none.

;