Difference between revisions of "Part:BBa K3002103"
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<partinfo>BBa_K3002103 short</partinfo>
 
<partinfo>BBa_K3002103 short</partinfo>
  
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This composite part contains the PAR-promoter (BBa_K3002027) in combination with the RPL23-Terminator (BBa_K3002006), a HA-tag (BBa_K3002017) and the coding sequence of the wildtype PETase (BBa_K3002004) plus the MoClo connectors for positions B2-B3 (BBa_K3002303), B4-B5 (BBa_K3002304) and B5-B6 (BBa_K3002305).  
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This composite part contains the PAR-promoter (<a href="https://parts.igem.org/Part:BBa_K3002027">BBa_K3002027</a>) in combination with the RPL23-Terminator (<a href="https://parts.igem.org/Part:BBa_K3002006">BBa_K3002006</a>), a HA-tag (<a href="https://parts.igem.org/Part:BBa_K3002017">BBa_K3002017</a>) and the coding sequence of the wildtype PETase (<a href="https://parts.igem.org/Part:BBa_K3002004">BBa_K3002004</a>) plus the MoClo connectors for positions B2-B3 (<a href="https://parts.igem.org/Part:BBa_K3002303">BBa_K3002303</a>), B4-B5 (<a href="https://parts.igem.org/Part:BBa_K3002304">BBa_K3002304</a>) and B5-B6 (<a href="https://parts.igem.org/Part:BBa_K3002305">BBa_K3002305</a>).  
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 23:51, 21 October 2019


Level 1 Wildtype PETase + His (Phytobrick)

This composite part contains the PAR-promoter (BBa_K3002027) in combination with the RPL23-Terminator (BBa_K3002006), a HA-tag (BBa_K3002017) and the coding sequence of the wildtype PETase (BBa_K3002004) plus the MoClo connectors for positions B2-B3 (BBa_K3002303), B4-B5 (BBa_K3002304) and B5-B6 (BBa_K3002305).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1002
    Illegal PstI site found at 1975
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 249
    Illegal PstI site found at 1002
    Illegal PstI site found at 1975
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1002
    Illegal PstI site found at 1975
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1002
    Illegal PstI site found at 1975
    Illegal NgoMIV site found at 737
    Illegal NgoMIV site found at 764
    Illegal NgoMIV site found at 2415
  • 1000
    COMPATIBLE WITH RFC[1000]