Difference between revisions of "Part:BBa K3228040"
 
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specific text here
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NanoLuc is a small luminescent reporter with just a molecular weight of 19,5 kDA. This reporter stands out with a signal strength that is orders of magnitude higher than compared traditional luminescent reporters.
  
 
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<br>
 
<br>
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This part is contained in a collection of reporters for Synechococcus elongatus UTEX2973 from <a href="https://2019.igem.org/Team:Marburg">iGEM Marburg 2019.</a> They are specifically chosen for cyanobacteria as they show a different absorption and excitation behaviour compared to other bacterial cells due to their photosystems.Hence not every fluorescence based reporter can be recommended for the use in cyanobacteria. This set should be seen as a recommendation of reporters based on
+
This part is contained in a collection of reporters for <i>Synechococcus elongatus</i> UTEX2973 from <a href="https://2019.igem.org/Team:Marburg">iGEM Marburg 2019.</a> They are specifically chosen for cyanobacteria as they show a different absorption and excitation behaviour compared to other bacterial cells due to their photosystems. Hence not every fluorescence based reporter can be recommended for the use in cyanobacteria. This set should be seen as a recommendation of reporters based on
−
<a href="https://2019.igem.org/Team:Marburg/Results">experimental data</a>and literature. They are either fluorescent reporters that bypass the wavelength at which cyanobacteria absorb and emit light or luminescent reporters that show a greater signal strength over background signal.
+
<a href="https://2019.igem.org/Team:Marburg/Results">experimental data</a>and literature. They are either fluorescence reporters that bypass the wavelength at which cyanobacteria absorb and emit light or luminescent reporters that show a greater signal strength over background signal.
 
   
 
   
 
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These parts are compatible with the RCF [1000] standard. These parts are compatible with any Part Collection that uses the Phytobrick standard of overhangs. For more information we recommend to head over to <a href="http://2018.igem.org/Team:Marburg/Design"> Design of the Marburg Collection</a>.
+
These parts are compatible with the RCF [1000] standard and can be used in any part collection that uses the PhytoBrick standard of overhangs. For more information we recommend to head over to <a href="http://2018.igem.org/Team:Marburg/Design"> Design of the Marburg Collection</a>.
 
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===Parts of the green expansion===
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===Available reporters===
  
 
<div class="PCListIcon" style="display:flex;flex-direction:row; flex-wrap: nowrap; justify-content:space-evenly; align-items:flex-start;font-size:82%;">
 
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[[File:T--Marburg--5'Con.png|90px|thumb|none|
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[[File:Icon L-Reporter.png|90px|thumb|none|
  
 
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<li> <a href="https://parts.igem.org/Part:BBa_K3228000">K3228000 </a> (aNSo1 integration up) </li>
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<li> <a href="https://parts.igem.org/Part:BBa_K3228040">K3228040 </a> <br>(NanoLuc) </li>
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<li> <a href="https://parts.igem.org/Part:BBa_K3228001">K3228001 </a> (aNSo2 integration up) </li>
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<li> <a href="https://parts.igem.org/Part:BBa_K3228041">K3228041 </a> <br>(NanoLuc S.e.)</li>
−
<li> <a href="https://parts.igem.org/Part:BBa_K3228020">K3228020 </a> <br> (NS1 up) </li>
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<li> <a href="https://parts.igem.org/Part:BBa_K3228042">K3228042 </a> <br> (teLuc S.e.) </li>
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<li> <a href="https://parts.igem.org/Part:BBa_K3228021">K3228021 </a> <br> (NS2 up) </li>
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<li> <a href="https://parts.igem.org/Part:BBa_K3228043">K3228043 </a> <br> (Antares S.e.) </li>
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<li> <a href="https://parts.igem.org/Part:BBa_K3228022">K3228022 </a> <br> (NS3 up) </li>
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−
 
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</b>
 
</b>
 
</ul>
 
</ul>
 
</html>]]
 
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[[File:T--Marburg--Terminator.png|90px|thumb|none|
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[[File:Icon F-Reporter.png|90px|thumb|none|
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    <li> <a href="https://parts.igem.org/Part:BBa_K3228004">K3228004 </a> (shortTB0010) </li>
 
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    <li> <a href="https://parts.igem.org/Part:BBa_K3228005">K3228005 </a> (shortTB0015) </li>
 
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    <li> <a href="https://parts.igem.org/Part:BBa_K3228006">K3228006 </a> (shortTB1002) </li>
 
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    <li> <a href="https://parts.igem.org/Part:BBa_K3228007">K3228007 </a> (shortTB1003) </li>
 
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    <li> <a href="https://parts.igem.org/Part:BBa_K3228008">K3228008 </a> (shortTB1004) </li>
 
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    <li> <a href="https://parts.igem.org/Part:BBa_K3228009">K3228009 </a> (shortTB1005) </li>
 
−
    <li> <a href="https://parts.igem.org/Part:BBa_K3228010">K3228010 </a> (shortTB1006) </li>
 
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    <li> <a href="https://parts.igem.org/Part:BBa_K3228011">K3228011 </a> (shortTB1007) </li>
 
−
    <li> <a href="https://parts.igem.org/Part:BBa_K3228012">K3228012 </a> (shortTB1008) </li>
 
−
    <li> <a href="https://parts.igem.org/Part:BBa_K3228013">K3228013 </a> (shortTB1009) </li>
 
−
    <li> <a href="https://parts.igem.org/Part:BBa_K3228014">K3228014 </a> (shortTB1010) </li>
 
−
    <li> <a href="https://parts.igem.org/Part:BBa_K3228015">K3228015 </a> (shortDummy) </li>
 
  
 +
<li> <a href="https://parts.igem.org/Part:BBa_K3228044">K3228044 </a> <br> (eYFP S.e.) </li>
 +
<li> <a href="https://parts.igem.org/Part:BBa_K3228045">K3228045 </a> <br> (phluorin2 S.e.) </li>
 +
<li> <a href="https://parts.igem.org/Part:BBa_K3228046">K3228046 </a> <br> (rxYFP S.e.) </li>
 +
<li> <a href="https://parts.igem.org/Part:BBa_K3228047">K3228047 </a> <br> (sfGFP S.e.) </li>
 +
<li> <a href="https://parts.igem.org/Part:BBa_K3228048">K3228048 </a> <br> (sfGFP S.e.) </li>
 +
<li> <a href="https://parts.igem.org/Part:BBa_K3228049">K3228049 </a> <br> (mTurqoise S.e.) </li>
 
</b>
 
</b>
 
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[[File:T--Marburg--3'Con.png|90px|thumb|none|<html>
 
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<ul> <b>
 
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<li> <a href="https://parts.igem.org/Part:BBa_K3228002">K3228002 </a> (aNSo1 integration down) </li>
 
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<li> <a href="https://parts.igem.org/Part:BBa_K3228003">K3228003 </a> (aNSo1 integration down) </li>
 
−
<li> <a href="https://parts.igem.org/Part:BBa_K3228023">K3228023 </a> <br> (NS1 down) </li>
 
−
<li> <a href="https://parts.igem.org/Part:BBa_K3228024">K3228024 </a> <br> (NS2 down) </li>
 
−
<li> <a href="https://parts.igem.org/Part:BBa_K3228025">K3228025 </a> <br> (NS3 down) </li>
 
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</ul>
 
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</b>
 
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[[File:T--Marburg--ORI.png|90px|thumb|none|<html>
 
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<ul> <b>
 
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    <li> <a href="https://parts.igem.org/Part:BBa_K3228026">K3228026 </a> (oriT integration) </li>
 
−
    <li> <a href="https://parts.igem.org/Part:BBa_K3228069">K3228069 </a> (panS SpecRes LVL1) </li>
 
−
    <li> <a href="https://parts.igem.org/Part:BBa_K3228089">K3228089 </a> (panS KanRes LVL2) </li>
 
−
 
−
</ul>
 
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</b>
 
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[[File:T--Marburg--Antibiotic_Resistance.png|90px|thumb|none|
 
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    <li><a href="https://parts.igem.org/Part:BBa_K3228016">K3228016 </a> (SpecRes_short) </li>
 
−
    <li><a href="https://parts.igem.org/Part:BBa_K3228017">K3228017 </a> <br> (CmlRes_short) </li>
 
−
    <li><a href="https://parts.igem.org/Part:BBa_K3228018">K3228018 </a> <br> (TetRes_short) </li>
 
−
    <li><a href="https://parts.igem.org/Part:BBa_K3228019">K3228019 </a> <br> (KanRes_short) </li>
 
−
    <li><a href="https://parts.igem.org/Part:BBa_K3228027">K3228027 </a> <br> (GenRes_short) </li>
 
−
 
−
</ul>
 
−
</b>
 
 
</html>]]
 
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</div>
 
</div>

Latest revision as of 23:44, 21 October 2019


pMC_0_4_22_NanoLuc-K1159001

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 19
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
    Illegal AgeI site found at 520
  • 1000
    COMPATIBLE WITH RFC[1000]

Description

NanoLuc is a small luminescent reporter with just a molecular weight of 19,5 kDA. This reporter stands out with a signal strength that is orders of magnitude higher than compared traditional luminescent reporters.

This part is contained in a collection of reporters for Synechococcus elongatus UTEX2973 from iGEM Marburg 2019. They are specifically chosen for cyanobacteria as they show a different absorption and excitation behaviour compared to other bacterial cells due to their photosystems. Hence not every fluorescence based reporter can be recommended for the use in cyanobacteria. This set should be seen as a recommendation of reporters based on experimental dataand literature. They are either fluorescence reporters that bypass the wavelength at which cyanobacteria absorb and emit light or luminescent reporters that show a greater signal strength over background signal.

Compability

These parts are compatible with the RCF [1000] standard and can be used in any part collection that uses the PhytoBrick standard of overhangs. For more information we recommend to head over to Design of the Marburg Collection.

Available reporters