Difference between revisions of "Part:BBa K3117015:Design"
Lena Schorr (Talk | contribs) |
|||
| Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | + | ||
| + | Codon optimization was performed for mammalian expression. | ||
| Line 13: | Line 14: | ||
===Source=== | ===Source=== | ||
| − | + | SpyTag origins from genomic DNA. | |
===References=== | ===References=== | ||
| + | |||
| + | 1. Hatlem, Daniel; Trunk, Thomas; Linke, Dirk; Leo, Jack C. (2019): Catching a SPY: Using the SpyCatcher-SpyTag and Related Systems for Labeling and Localizing Bacterial Proteins. In: International journal of molecular sciences 20 (9). DOI: 10.3390/ijms20092129. | ||
Revision as of 23:39, 21 October 2019
SpyTag codon optimized for CHO expression
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Codon optimization was performed for mammalian expression.
Source
SpyTag origins from genomic DNA.
References
1. Hatlem, Daniel; Trunk, Thomas; Linke, Dirk; Leo, Jack C. (2019): Catching a SPY: Using the SpyCatcher-SpyTag and Related Systems for Labeling and Localizing Bacterial Proteins. In: International journal of molecular sciences 20 (9). DOI: 10.3390/ijms20092129.