Difference between revisions of "Part:BBa K2983005"
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This part is the Ura3 auxotrophic selection marker for ''Yarrowia lipolytica''.
 
This part is the Ura3 auxotrophic selection marker for ''Yarrowia lipolytica''.
  
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The URA3 auxotrophic selection marker (BBa_K2983005) which is composed of the URA3 promoter (BBa_K2983002), URA3 gene (BBa_K2983003) and the URA3 terminator (BBa_K2983004). The URA3 gene encodes the orotidine 5'-phosphate decarboxylase, an enzyme (EC. 4.1.1.23) that catalyzes the decarboxylation of orotidine monophosphate to uridine monophosphate in the pyrimidine ribonucleotide synthesis pathway.
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===Usage and Biology===
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The URA3 auxotrophic selection marker ([[Part:BBa_K2983005|BBa_K2983005]]) is composed of the URA3 promoter ([[Part:BBa_K2983002|BBa_K2983002]]), URA3 gene ([[Part:BBa_K2983003|BBa_K2983003]]) and the URA3 terminator ([[Part:BBa_K2983004|BBa_K2983004]]).  
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The Ura3 gene encodes the orotidine 5'-phosphate decarboxylase, an enzyme (EC. 4.1.1.23) that catalyzes the decarboxylation of orotidine monophosphate to uridine monophosphate in the pyrimidine ribonucleotides synthesis pathway. In the absence of this enzyme, the cells are able to grow only if uracil or uridine are present in the media.
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It is a wildly used auxotrophic selection marker in yeast.
  
 
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===Usage and Biology===
 
===Usage and Biology===
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Latest revision as of 23:00, 21 October 2019


Ura3 auxotrophic selection marker

This part is the Ura3 auxotrophic selection marker for Yarrowia lipolytica.

Usage and Biology

The URA3 auxotrophic selection marker (BBa_K2983005) is composed of the URA3 promoter (BBa_K2983002), URA3 gene (BBa_K2983003) and the URA3 terminator (BBa_K2983004).

The Ura3 gene encodes the orotidine 5'-phosphate decarboxylase, an enzyme (EC. 4.1.1.23) that catalyzes the decarboxylation of orotidine monophosphate to uridine monophosphate in the pyrimidine ribonucleotides synthesis pathway. In the absence of this enzyme, the cells are able to grow only if uracil or uridine are present in the media.

It is a wildly used auxotrophic selection marker in yeast.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 195
    Illegal XhoI site found at 228
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 383
    Illegal AgeI site found at 486
  • 1000
    COMPATIBLE WITH RFC[1000]