Difference between revisions of "Part:BBa K3192018"
 
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<p>This part contains the gene styD which encodes for the protein phenylacetaldehyde dehydrogenase. This gene was obtained from <i>Pseudomonas putida</i> S12, and combines in conjunction with the overall cluster styABCDE. This is the final step in the degradation of styrene to phenylacetic acid. This catalyzes the oxidation  of phenylacetaldehyde to phenylacetic acid.<sup>1</sup> </p>  
 
<p>This part contains the gene styD which encodes for the protein phenylacetaldehyde dehydrogenase. This gene was obtained from <i>Pseudomonas putida</i> S12, and combines in conjunction with the overall cluster styABCDE. This is the final step in the degradation of styrene to phenylacetic acid. This catalyzes the oxidation  of phenylacetaldehyde to phenylacetic acid.<sup>1</sup> </p>  
 
[[File:T--Virginia--styDprotein.png|500px|thumb|left|Protein structure of phenylacetaldehyde dehydrogenase. Figure acquired from Structure and biochemistry of phenylacetaldehyde dehydrogenase from the Pseudomonas putida S12 styrene catabolic pathway.<sup>1</sup>]]
 
[[File:T--Virginia--styDprotein.png|500px|thumb|left|Protein structure of phenylacetaldehyde dehydrogenase. Figure acquired from Structure and biochemistry of phenylacetaldehyde dehydrogenase from the Pseudomonas putida S12 styrene catabolic pathway.<sup>1</sup>]]
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<h3>References: </h3>
 
<h3>References: </h3>
 
<p> 1) Crabo, A. G. et al.Structure and biochemistry of phenylacetaldehyde dehydrogenase from the Pseudomonas putida S12 styrene catabolic pathway. <i>Archives of Biochemistry and Biophysics</i> 616,47–58 (2017). </p>  
 
<p> 1) Crabo, A. G. et al.Structure and biochemistry of phenylacetaldehyde dehydrogenase from the Pseudomonas putida S12 styrene catabolic pathway. <i>Archives of Biochemistry and Biophysics</i> 616,47–58 (2017). </p>  
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===Usage and Biology===
 
===Usage and Biology===
  
 
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3192018 SequenceAndFeatures</partinfo>
  
  

Latest revision as of 22:59, 21 October 2019


styD

This part contains the gene styD which encodes for the protein phenylacetaldehyde dehydrogenase. This gene was obtained from Pseudomonas putida S12, and combines in conjunction with the overall cluster styABCDE. This is the final step in the degradation of styrene to phenylacetic acid. This catalyzes the oxidation of phenylacetaldehyde to phenylacetic acid.1

Protein structure of phenylacetaldehyde dehydrogenase. Figure acquired from Structure and biochemistry of phenylacetaldehyde dehydrogenase from the Pseudomonas putida S12 styrene catabolic pathway.1




























References:

1) Crabo, A. G. et al.Structure and biochemistry of phenylacetaldehyde dehydrogenase from the Pseudomonas putida S12 styrene catabolic pathway. Archives of Biochemistry and Biophysics 616,47–58 (2017).



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1380
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 540
    Illegal NgoMIV site found at 907
    Illegal AgeI site found at 730
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1318