Difference between revisions of "Part:BBa K3037004"
 
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|'''RFC standard'''
 
|'''RFC standard'''
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|RFC 25 compatible
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|[[Help:Assembly_standard_25|Freiburg RFC25 standard]]
 
|-
 
|-
 
|'''Backbone'''
 
|'''Backbone'''
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|pSB1C3<br>  
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|[https://parts.igem.org/Help:2019_DNA_Distribution pSB1C3<br>]
 
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|-
 
|'''Submitted by'''
 
|'''Submitted by'''
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|Team:TU_Dresden 2019[https://2019.igem.org/Team:TU_Dresden]
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|Team: [https://2019.igem.org/Team:TU_Dresden TU_Dresden 2019]
 
|}
 
|}
  
  
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=== Overview ===
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== Overview ==
  
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The TU Dresden team 2019 used this linker in order to make their fusion protein [https://parts.igem.org/Part:BBa_K3037003 BBa_K3037003].
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The TU Dresden team 2019 used this linker to make the fusion proteins [https://parts.igem.org/Part:BBa_K3037005 (BBa_K3037005)] and [https://parts.igem.org/Part:BBa_K3037003 (BBa_K3037003)].
  
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== Characterization ==
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This part, encodes for a linker used in between translationally fused proteins. The amino acid composition is "GSAGSAAGSG". The high amounts of glycine and serine residues make it a very flexible linker.
  
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<!-- Add more about the biology of this part here
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== Sequence ==
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===Usage and Biology===
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This BioBrick is flanked by the RFC25 standard.
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=== Sequence ===
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<partinfo>BBa_K3037004 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3037004 SequenceAndFeatures</partinfo>
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<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Latest revision as of 22:23, 21 October 2019

Linker

Linker
Function Linker for fusion proteins
Use in Escherichia coli
RFC standard Freiburg RFC25 standard
Backbone pSB1C3
Submitted by Team: TU_Dresden 2019


Overview

The TU Dresden team 2019 used this linker to make the fusion proteins (BBa_K3037005) and (BBa_K3037003).

Characterization

This part, encodes for a linker used in between translationally fused proteins. The amino acid composition is "GSAGSAAGSG". The high amounts of glycine and serine residues make it a very flexible linker.

Sequence

This BioBrick is flanked by the RFC25 standard.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]