Difference between revisions of "Part:BBa K3042006"

Revision as of 22:21, 21 October 2019

Dino III RFP Plasmid

DinoIII is a dinoflagellate expression system that was constructed on the basis of genomic and transcriptic data from many dinoflagellates and created by the University of Connecticut. DinoIII- RFP is a dinoflagellate optimized reporter plasmid that contains a red fluorescence protein. Designed by GSU’s iGEM team, it will be used to transform into the dinoflagellate, O.Marina. The original Dino III vector included a green fluorescence protein which for O.marina, a heterotrophic dinoflagellate, is not an issue. However, because we plan to transform Symbiodinium with this composite part, RFP was designated to replace GFP to prevent inhibition of the Symbiodinium’s photosynthetic abilities.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 693
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1764
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1376
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 693
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 693
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1746

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3042006 short</partinfo>
-A dinoflagellate optimized reporter plasmid.
+DinoIII is a dinoflagellate expression system that was constructed on the basis of genomic and transcriptic data from many dinoflagellates and created by the University of Connecticut. DinoIII- RFP is a dinoflagellate optimized reporter plasmid that contains a red fluorescence protein. Designed by GSU’s iGEM team, it will be used to transform into the dinoflagellate, O.Marina. The original Dino III vector included a green fluorescence protein which for O.marina, a heterotrophic dinoflagellate, is not an issue. However, because we plan to transform Symbiodinium with this composite part, RFP was designated to replace GFP to prevent inhibition of the Symbiodinium’s photosynthetic abilities.
 <!-- Add more about the biology of this part here