Difference between revisions of "Part:BBa K3037004"
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This BioBrick was used to make the fusion proteins [https://parts.igem.org/Part:BBa_K3037005 (BBa_K3037005)] and [https://parts.igem.org/Part:BBa_K3037003 (BBa_K3037003)] as well. | This BioBrick was used to make the fusion proteins [https://parts.igem.org/Part:BBa_K3037005 (BBa_K3037005)] and [https://parts.igem.org/Part:BBa_K3037003 (BBa_K3037003)] as well. | ||
| − | == | + | == Characterization == |
| − | This part, encodes for a linker used in between translationally fused proteins. The amino acid composition is "GSAGSAAGSG". The high amounts of glycine and serine residues make it a very flexible linker. | + | This part, encodes for a linker used in between translationally fused proteins. The amino acid composition is "GSAGSAAGSG". The high amounts of glycine and serine residues make it a very flexible linker. |
== Sequence == | == Sequence == | ||
Revision as of 22:12, 21 October 2019
Linker
| Linker | |
|---|---|
| Function | Linker for fusion proteins |
| Use in | Escherichia coli |
| RFC standard | Freiburg RFC25 standard |
| Backbone | pSB1C3 |
| Submitted by | Team: TU_Dresden 2019 |
Overview
The TU Dresden team 2019 used this linker in order to make their fusion protein BBa_K3037003 (more information).
This BioBrick was used to make the fusion proteins (BBa_K3037005) and (BBa_K3037003) as well.
Characterization
This part, encodes for a linker used in between translationally fused proteins. The amino acid composition is "GSAGSAAGSG". The high amounts of glycine and serine residues make it a very flexible linker.
Sequence
This BioBrick is flanked by the RFC25 standard.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]