Difference between revisions of "Part:BBa K3183003"

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<partinfo>BBa_K3183003 short</partinfo>
 
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Putative AgrA2 Promoter for binding of AgrA2 transcription factor from AgrAC2 two-component quorum sensing system from <i>C. difficile</i>
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Putative AgrA2 Promoter for binding of AgrA2 transcription factor from AgrAC2 two-component quorum sensing system from <i>C. difficile</i>. The promoter is located in the agr operon and regulates <i>agrACDB</i> transcription in response to Quorum sensing in <i>C. difficile</i>.<sup>1</sup>
  
 
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===Use by Oxford 2019===
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===Use by Team Oxford 2019===
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This part was used in two composite parts in Oxford 2019's ProQuorum project, for detecting <i>C. difficile</i> and lysing it in response using the CD27L endolysin: [https://parts.igem.org/Part:BBa_K3183104 BBa_K3183104] and [https://parts.igem.org/Part:BBa_K3183300 BBa_K3183300].
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This part was used in two composite parts for detecting <i>C. difficile</i> and lysing it in response using the CD27L endolysin: <partinfo>BBa_K3183104</partinfo> and <partinfo>BBa_K3183300</partinfo>.
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===Reference===
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1. Martin, Melissa J., et al. “The Agr Locus Regulates Virulence and Colonization Genes in Clostridium Difficile 027.” Journal of Bacteriology, vol. 195, no. 16, Aug. 2013, pp. 3672–81. PubMed, doi:10.1128/JB.00473-13.

Latest revision as of 22:05, 21 October 2019


Putative Promoter Regulated by AgrA2 in C. difficile

Putative AgrA2 Promoter for binding of AgrA2 transcription factor from AgrAC2 two-component quorum sensing system from C. difficile. The promoter is located in the agr operon and regulates agrACDB transcription in response to Quorum sensing in C. difficile.1

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Use by Team Oxford 2019

This part was used in two composite parts for detecting C. difficile and lysing it in response using the CD27L endolysin: BBa_K3183104 and BBa_K3183300.

Reference

1. Martin, Melissa J., et al. “The Agr Locus Regulates Virulence and Colonization Genes in Clostridium Difficile 027.” Journal of Bacteriology, vol. 195, no. 16, Aug. 2013, pp. 3672–81. PubMed, doi:10.1128/JB.00473-13.