Difference between revisions of "Part:BBa K2984020"
 
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This part is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. For <i>in vivo</i> detection of proteins in  <i>C. reinhardtii</i> or its surroundings. The green fluorescent protein variant carries amino acid substitutions that shift the protein to which it is bound to an emission peak at 530 nm seen as yellow (Ormo et al., 1996). For detection of fluorescence it is not necessary to process a sample nor adding a substance to make it visible making it safe for <i>C. reinhardtii</i> as it can be detected with a fluorescence microscop
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This part is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. Yellow fluorescent protein mVenus for <i>in vivo</i> detection of proteins in  <i>C. reinhardtii</i> or its surroundings. The YFP mVenus has an excitation peak at 515 nm and an emission peak at 528 nm (Kremers et al. 2006).  
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This part was designed to be used with the MoClo standard and has B5-B5 overhangs.
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K2984020 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2984020 SequenceAndFeatures</partinfo>
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===Usage and Biology===
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YFP containing <i>C. reinhardtii</i> can be observed under a fluorescence microscope to see its fluorescence. This part will make <i>C. reinhardtii</i> emit fluorescence at a wavelength of 528 nm. If you use this part you can expect fluorescence as seen on Fig. 1.
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<img src="https://2019.igem.org/wiki/images/1/1a/T--Humboldt_Berlin--YFP_klon.jpeg" alt="YFP-clone" width="500" />
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<figcaption>Fig. 1 - Fluorescent C. reinhardtii with our construct <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984019&action=edit">BBa_K2984019</a> under a fluorescent microscope. </figcaption>
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==Characterization==
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<img src="https://static.igem.org/mediawiki/parts/9/98/T--Humboldt_Berlin--L0-YFP_B5-B5.png" alt="Plate_L0-YFP_B5-B5_E.coli" width="500">
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<figcaption>Fig.1 - Successful transformation of L0-YFP_B5-B5 into <i>E. coli</i>.</figcaption>
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<partinfo>BBa_K2984020 parameters</partinfo>
 
<partinfo>BBa_K2984020 parameters</partinfo>
 
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==References==
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Kremers, G. J., Goedhart, J., van Munster, E. B., & Gadella, T. W. (2006). Cyan and yellow super fluorescent proteins with improved brightness, protein folding, and FRET Förster radius. Biochemistry, 45(21), 6570-6580.
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Latest revision as of 21:59, 21 October 2019


YFP - Yellow fluorescent protein for detection B5-B5

This part is a part of the Chlamy-HUB-Collection. Yellow fluorescent protein mVenus for in vivo detection of proteins in C. reinhardtii or its surroundings. The YFP mVenus has an excitation peak at 515 nm and an emission peak at 528 nm (Kremers et al. 2006).

This part was designed to be used with the MoClo standard and has B5-B5 overhangs.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 220
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 220
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 220
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 220
    Illegal NgoMIV site found at 697
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

YFP containing C. reinhardtii can be observed under a fluorescence microscope to see its fluorescence. This part will make C. reinhardtii emit fluorescence at a wavelength of 528 nm. If you use this part you can expect fluorescence as seen on Fig. 1. YFP-clone

Fig. 1 - Fluorescent C. reinhardtii with our construct BBa_K2984019 under a fluorescent microscope.

Characterization

Plate_L0-YFP_B5-B5_E.coli
Fig.1 - Successful transformation of L0-YFP_B5-B5 into E. coli.


References

  1. Kremers, G. J., Goedhart, J., van Munster, E. B., & Gadella, T. W. (2006). Cyan and yellow super fluorescent proteins with improved brightness, protein folding, and FRET Förster radius. Biochemistry, 45(21), 6570-6580.