Difference between revisions of "Part:BBa K3095003"

 
 
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<partinfo>BBa_K3095003 short</partinfo>
 
<partinfo>BBa_K3095003 short</partinfo>
  
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Utilizing the parts from Jesus & Voigt et al 2017 work and the parts from UCAS-China 2018 iGEM team, we built the blue light sensor independent from the red and the green light.
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This circuit utilises the blue light sensor YF1 together with fixJ for detect the light, regulating the PhIF regulated by the promoter PfixK2 fixJ-regulated. The PhIF regulates the sigma fragment T3 which is part of the T7 RNAP core. We reconstructed this part for being able to induces only the blue light, independent from the red and green regulations, since the entire regulation is originally spread across different plasmids.
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This was constructed based in the parts from Jesus & Voigt et al 2017, that went characterised and submitted in the registry by the UCAS-China 2018 iGEM team.
  
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===Usage and Biology===
 
===Usage and Biology===
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Utilizing the plasmids from Jesus & Voigt et al 2017 we reconstructed only the blue light detector, therefore being able to be regulated in a single construct.
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This circuit can be used with the part BBa_K2598026 for induction controlled only by blue led light.
  
 
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Latest revision as of 21:57, 21 October 2019


Blue light regulated inducer circuit

This circuit utilises the blue light sensor YF1 together with fixJ for detect the light, regulating the PhIF regulated by the promoter PfixK2 fixJ-regulated. The PhIF regulates the sigma fragment T3 which is part of the T7 RNAP core. We reconstructed this part for being able to induces only the blue light, independent from the red and green regulations, since the entire regulation is originally spread across different plasmids. This was constructed based in the parts from Jesus & Voigt et al 2017, that went characterised and submitted in the registry by the UCAS-China 2018 iGEM team.

Usage and Biology

Utilizing the plasmids from Jesus & Voigt et al 2017 we reconstructed only the blue light detector, therefore being able to be regulated in a single construct. This circuit can be used with the part BBa_K2598026 for induction controlled only by blue led light.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2135
    Illegal NheI site found at 2158
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3988
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4786
    Illegal NgoMIV site found at 4858
    Illegal NgoMIV site found at 4948
    Illegal NgoMIV site found at 4966
    Illegal NgoMIV site found at 5458
    Illegal NgoMIV site found at 5751
    Illegal NgoMIV site found at 5845
    Illegal AgeI site found at 4500
    Illegal AgeI site found at 5626
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5515
    Illegal BsaI.rc site found at 4399