Difference between revisions of "Part:BBa K1741014:Experience"
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We characterized the ProD-sfGFP part (Bba_K1741014) by cloning this part into pZA, pZE and pZS backbone. We obtained the fluorescence data by measuring the fluorescence level for each 30 minutes for 6 hours. Also, we showed the growth curve of the cell containing the different origin plasmids with the different strains. We did not observe any significant differences between different strains within the context of fluorescence and OD. RFU/OD graphics illustrates the relative fluorescence units per optical density which depicts the fluoresce which are expressed in a certain unit relatively to cell growth.
 
We characterized the ProD-sfGFP part (Bba_K1741014) by cloning this part into pZA, pZE and pZS backbone. We obtained the fluorescence data by measuring the fluorescence level for each 30 minutes for 6 hours. Also, we showed the growth curve of the cell containing the different origin plasmids with the different strains. We did not observe any significant differences between different strains within the context of fluorescence and OD. RFU/OD graphics illustrates the relative fluorescence units per optical density which depicts the fluoresce which are expressed in a certain unit relatively to cell growth.
  
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https://static.igem.org/mediawiki/parts/c/cb/T--Bilkent-UNAMBG--pzsProD.png
  
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https://static.igem.org/mediawiki/parts/f/fc/T--Bilkent-UNAMBG--WT.png
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tp://parts.igem.org/wiki/images/c/c2/T--Bilkent-UNAMBG--pzaProD.png
  
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https://static.igem.org/mediawiki/parts/1/15/T--Bilkent-UNAMBG--PZEpro.png
  
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'''Figure 1:'''BL21 and DH5a(MG1655) OD Measurements
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https://static.igem.org/mediawiki/parts/f/fc/T--Bilkent-UNAMBG--WT.png
  
  
  
  
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https://static.igem.org/mediawiki/parts/1/15/T--Bilkent-UNAMBG--PZEpro.png
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'''Figure 3:''' pZe proD sfGFP RFU/OD Measurements
 
  
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https://static.igem.org/mediawiki/parts/c/c2/T--Bilkent-UNAMBG--pzaProD.png
 
  
  
  
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'''Figure 4:''' pZa proD sfGFP RFU/OD Measurements
 
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https://static.igem.org/mediawiki/parts/c/cb/T--Bilkent-UNAMBG--pzsProD.png
 
  
  

Revision as of 21:45, 21 October 2019


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Applications of BBa_K1741014

Bilkent-UNAMBG 2019 iGEM Team

We characterized the ProD-sfGFP part (Bba_K1741014) by cloning this part into pZA, pZE and pZS backbone. We obtained the fluorescence data by measuring the fluorescence level for each 30 minutes for 6 hours. Also, we showed the growth curve of the cell containing the different origin plasmids with the different strains. We did not observe any significant differences between different strains within the context of fluorescence and OD. RFU/OD graphics illustrates the relative fluorescence units per optical density which depicts the fluoresce which are expressed in a certain unit relatively to cell growth.

T--Bilkent-UNAMBG--pzsProD.png

tp://parts.igem.org/wiki/images/c/c2/T--Bilkent-UNAMBG--pzaProD.png

T--Bilkent-UNAMBG--PZEpro.png

T--Bilkent-UNAMBG--WT.png



ht





Figure 5: pZs proD sfGFP RFU/OD Measurements


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UNIQe187191167cf1c35-partinfo-00000000-QINU UNIQe187191167cf1c35-partinfo-00000001-QINU