Difference between revisions of "Part:BBa K3183002:Design"
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<partinfo>BBa_K3183002 short</partinfo> | <partinfo>BBa_K3183002 short</partinfo> | ||
| − | The part was identified in the article by Lizier et al; the gene was codon optimised for L. reuteri and synthesized by IDT. The part was leter assembled by Gibson Assembly into the composite part BBa_K3183101, which was finally assembled into the pTRKH3 vector (BBa_K3183050), again by Gibson Assembly | + | The part was identified in the article by Lizier et al; the gene was codon optimised for <i>L. reuteri</i> and synthesized by IDT. The part was leter assembled by Gibson Assembly into the composite part <partinfo>BBa_K3183101</partinfo>, which was finally assembled into the pTRKH3 vector (<partinfo>BBa_K3183050</partinfo>), again by Gibson Assembly |
<partinfo>BBa_K3183002 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3183002 SequenceAndFeatures</partinfo> | ||
Latest revision as of 21:35, 21 October 2019
Lactate Dehydrogenase Constitutive Promoter for Lactobacillus sp.
The part was identified in the article by Lizier et al; the gene was codon optimised for L. reuteri and synthesized by IDT. The part was leter assembled by Gibson Assembly into the composite part BBa_K3183101, which was finally assembled into the pTRKH3 vector (BBa_K3183050), again by Gibson Assembly
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 219
Design Notes
This promoter was specifically selected as an ideal promoter for L. reuteri because it conformed to the RF10, RF12, RF21, RF23 and RF25 standards.
Source
The promoter is derived from the lactate dehydrogenase (ldlL) promoter from Lactobacillus acidophilus.
References
Lizier, Michela, et al. “Comparison of Expression Vectors in Lactobacillus Reuteri Strains.” FEMS Microbiology Letters, vol. 308, no. 1, Aug. 2010, pp. 8–15., doi:10.1111/j.1574-6968.2010.01978.x.