Difference between revisions of "Part:BBa K3183002"
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| − | The ldhL promoter is a constitutive promoter that has been used in <i> Lactobacillus reuteri </i> in our composite part <partinfo>BBa_K3183100</partinfo>. | + | The ldhL promoter is a constitutive promoter that has been used in <i> Lactobacillus reuteri </i> in our composite part <partinfo>BBa_K3183100</partinfo>. |
===Characterisation=== | ===Characterisation=== | ||
Revision as of 21:27, 21 October 2019
Lactate Dehydrogenase Constitutive Promoter for Lactobacillus sp.
P-slp is a constitutive promoter which can be used in Lactobacillus reuteri 10023C, and may have uses in other Lactobacillus species.
This is a constitutive promoter from the lactate dehydrogenase (ldhL) promoter from Lactobacillus acidophilus1. It has been subsequently characterised in six strains of Lactobacillus reuteri and Lactococcus lactis spp. cremoris MG13632.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Use by Team Oxford 2019
The ldhL promoter is a constitutive promoter that has been used in Lactobacillus reuteri in our composite part BBa_K3183100.
Characterisation
The part was characterised in the composite part BBa_K3183101.
References
1. Kim, S F et al. “Cloning and nucleotide sequence of the Lactobacillus casei lactate dehydrogenase gene.” Applied and environmental microbiology vol. 57,8 (1991): 2413-7.
2. Lizier, Michela, et al. “Comparison of Expression Vectors in Lactobacillus Reuteri Strains.” FEMS Microbiology Letters, vol. 308, no. 1, 2010, pp. 8–15., doi:10.1111/j.1574-6968.2010.01978.x.