Difference between revisions of "Part:BBa K3174008:Design"
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The Austin_UTexas 2015 iGEM team originally identified that the SYFP2 gene was especially prone to IS10 element mutations (See their work <html><a href="http://2015.igem.org/Team:Austin_UTexas/Project/Plasmid_Study">here</a></html>). Kelsey Hu then performed the initial cloning and characterization of the redesigned sequence. | The Austin_UTexas 2015 iGEM team originally identified that the SYFP2 gene was especially prone to IS10 element mutations (See their work <html><a href="http://2015.igem.org/Team:Austin_UTexas/Project/Plasmid_Study">here</a></html>). Kelsey Hu then performed the initial cloning and characterization of the redesigned sequence. | ||
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| − | Later the Austin_UTexas 2019 iGEM team retrieved the sequence and Anna Bardenhagen performed propagation experiments replicating Austin_UTexas 2015's <html><a href="http://2015.igem.org/Team:Austin_UTexas/Project/Strain_Study">procedure</a></html> for both the original and redesigned SYFP2 sequences. This data was then used to fully characterize the stability of the new part | + | Later the Austin_UTexas 2019 iGEM team retrieved the sequence and Anna Bardenhagen performed propagation experiments replicating Austin_UTexas 2015's <html><a href="http://2015.igem.org/Team:Austin_UTexas/Project/Strain_Study">procedure</a></html> for both the original and redesigned SYFP2 sequences. This data was then used to fully characterize the stability of the new part as it compared to the original. A registry page was created to document the sequence and characterization of the new part. |
===Design Notes=== | ===Design Notes=== | ||
| − | The redesigned sequence must be less prone to IS10 insertion element mutations while still mainting the same amino acid sequence. | + | The redesigned sequence must be less prone to IS10 insertion element mutations while still mainting the same amino acid sequence. This was achieved by altering the sequence at positions 30-41 from <html>aggcgtagtaccg to <b>t</b>gg<b>g</b>gt<b>t</b>gt<b>t</b>cc<b>a</b>.</html> |
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===Source=== | ===Source=== | ||
Latest revision as of 21:26, 21 October 2019
SYFP2 coding sequence with IS hotspot removed
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Conception and Design
The Austin_UTexas 2015 iGEM team originally identified that the SYFP2 gene was especially prone to IS10 element mutations (See their work here). Kelsey Hu then performed the initial cloning and characterization of the redesigned sequence.
Later the Austin_UTexas 2019 iGEM team retrieved the sequence and Anna Bardenhagen performed propagation experiments replicating Austin_UTexas 2015's procedure for both the original and redesigned SYFP2 sequences. This data was then used to fully characterize the stability of the new part as it compared to the original. A registry page was created to document the sequence and characterization of the new part.
Design Notes
The redesigned sequence must be less prone to IS10 insertion element mutations while still mainting the same amino acid sequence. This was achieved by altering the sequence at positions 30-41 from aggcgtagtaccg to tggggttgttcca.
Source
The original SYFP2 coding sequence (BBa_K864100)