Difference between revisions of "Part:BBa K3183001:Design"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K3183001 short</partinfo>
 
<partinfo>BBa_K3183001 short</partinfo>
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The part was identified in the article by Lizier et al; the gene was codon optimised for <i>L. reuteri</i> and synthesized by IDT. The part was leter assembled by Gibson Assembly into <partinfo>BBa_K3183102</partinfo>, which was finally assembled into the pTRKH3 vector (<partinfo>BBa_K3183050</partinfo>), again by Gibson Assembly
  
 
<partinfo>BBa_K3183001 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3183001 SequenceAndFeatures</partinfo>
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===Design Notes===
 
===Design Notes===
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n/a
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This promoter was specifically selected as an ideal promoter for L. reuteri because it conformed to the RF10, RF12, RF21, RF23, RF25, and RF1000 standards
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−
 
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===Source===
 
===Source===
  
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It is derived from the L.acidophilus surface layer protein promoter.  
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It is derived from the <i>L. acidophilus</i> surface layer protein promoter.
  
 
===References===
 
===References===
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Lizier, Michela, et al. “Comparison of Expression Vectors in Lactobacillus Reuteri  Strains.” FEMS Microbiology Letters, vol. 308, no. 1, Aug. 2010, pp. 8–15., doi:10.1111/j.1574-6968.2010.01978.x.

Latest revision as of 21:23, 21 October 2019


Surface (S)-Layer Protein Constitutive Promoter for Lactobacillus sp.

The part was identified in the article by Lizier et al; the gene was codon optimised for L. reuteri and synthesized by IDT. The part was leter assembled by Gibson Assembly into BBa_K3183102, which was finally assembled into the pTRKH3 vector (BBa_K3183050), again by Gibson Assembly


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This promoter was specifically selected as an ideal promoter for L. reuteri because it conformed to the RF10, RF12, RF21, RF23, RF25, and RF1000 standards

Source

It is derived from the L. acidophilus surface layer protein promoter.

References

Lizier, Michela, et al. “Comparison of Expression Vectors in Lactobacillus Reuteri  Strains.” FEMS Microbiology Letters, vol. 308, no. 1, Aug. 2010, pp. 8–15., doi:10.1111/j.1574-6968.2010.01978.x.