Difference between revisions of "Part:BBa K3286008:Design"

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===References===
 
===References===
  
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<ol><li>Larson, M. H., Gilbert, L. A., Wang, X., Lim, W. A., Weissman, J. S., & Qi, L. S. (2013). CRISPR interference (CRISPRi) for sequence-specific control of gene expression. Nature protocols, 8(11), 2180.</li></ol>
+
<ol>
 +
<li>Qi, L. S., Larson, M. H., Gilbert, L. A., Doudna, J. A., Weissman, J. S., Arkin, A. P., & Lim, W. A. (2013). Repurposing CRISPR as an RNA-γuided platform for sequence-specific control of gene expression. Cell, 152(5), 1173–1183. https://doi.org/10.1016/j.cell.2013.02.022 </li>
 +
<li>Bikard, D., Jiang, W., Samai, P., Hochschild, A., Zhang, F., & Marraffini, L. A. (2013). Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system. Nucleic Acids Research, 41(15), 7429–7437. https://doi.org/10.1093/nar/gkt520 </li>
 +
<li>Zetsche, B., Gootenberg, J. S., Abudayyeh, O. O., Slaymaker, I. M., Makarova, K. S., Essletzbichler, P., … Zhang, F. (2015). Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System. Cell, 163(3), 759–771. https://doi.org/10.1016/j.cell.2015.09.038 </li>
 +
<li>Bondy-Denomy, J. et al. (2013) ‘Bacteriophage genes that inactivate the CRISPR/Cas bacterial immune system.’, Nature. England, 493(7432), pp. 429–432. doi: 10.1038/nature11723. </li>
 +
<li>Trasanidou, D., Gerós, A. S., Mohanraju, P., Nieuwenweg, A. C., Nobrega, F. L., & Staals, R. H. J. (2019). Keeping CRISPR in check: diverse mechanisms of phage-encoded anti-crisprs. FEMS Microbiology Letters, 366(9), 98. https://doi.org/10.1093/femsle/fnz098 </li>
 +
<li>Nakamura, M. et al. (2019) ‘Anti-CRISPR-mediated control of gene editing and synthetic circuits in eukaryotic cells’, Nature Communications, 10(1), p. 194. doi: 10.1038/s41467-018-08158-x.</li>
 +
<li>Larson, M. H., Gilbert, L. A., Wang, X., Lim, W. A., Weissman, J. S., & Qi, L. S. (2013). CRISPR interference (CRISPRi) for sequence-specific control of gene expression. Nature Protocols, 8(11), 2180–2196. https://doi.org/10.1038/nprot.2013.132 </li>
 +
<li>Farasat, I., Kushwaha, M., Collens, J., Easterbrook, M., Guido, M., & Salis, H. M. (2014). Efficient search, mapping, and optimization of multi‐protein genetic systems in diverse bacteria. Molecular systems biology, 10(6). </li>
 +
<li>Ng, C. Y., Farasat, I., Maranas, C. D., & Salis, H. M. (2015). Rational design of a synthetic Entner–Doudoroff pathway for improved and controllable NADPH regeneration. Metabolic engineering, 29, 86-96. </li>

Latest revision as of 21:17, 21 October 2019


IPTG inducible dCas9 expression module


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1200
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3479
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

/


Source

/

References

  1. Qi, L. S., Larson, M. H., Gilbert, L. A., Doudna, J. A., Weissman, J. S., Arkin, A. P., & Lim, W. A. (2013). Repurposing CRISPR as an RNA-γuided platform for sequence-specific control of gene expression. Cell, 152(5), 1173–1183. https://doi.org/10.1016/j.cell.2013.02.022
  2. Bikard, D., Jiang, W., Samai, P., Hochschild, A., Zhang, F., & Marraffini, L. A. (2013). Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system. Nucleic Acids Research, 41(15), 7429–7437. https://doi.org/10.1093/nar/gkt520
  3. Zetsche, B., Gootenberg, J. S., Abudayyeh, O. O., Slaymaker, I. M., Makarova, K. S., Essletzbichler, P., … Zhang, F. (2015). Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System. Cell, 163(3), 759–771. https://doi.org/10.1016/j.cell.2015.09.038
  4. Bondy-Denomy, J. et al. (2013) ‘Bacteriophage genes that inactivate the CRISPR/Cas bacterial immune system.’, Nature. England, 493(7432), pp. 429–432. doi: 10.1038/nature11723.
  5. Trasanidou, D., Gerós, A. S., Mohanraju, P., Nieuwenweg, A. C., Nobrega, F. L., & Staals, R. H. J. (2019). Keeping CRISPR in check: diverse mechanisms of phage-encoded anti-crisprs. FEMS Microbiology Letters, 366(9), 98. https://doi.org/10.1093/femsle/fnz098
  6. Nakamura, M. et al. (2019) ‘Anti-CRISPR-mediated control of gene editing and synthetic circuits in eukaryotic cells’, Nature Communications, 10(1), p. 194. doi: 10.1038/s41467-018-08158-x.
  7. Larson, M. H., Gilbert, L. A., Wang, X., Lim, W. A., Weissman, J. S., & Qi, L. S. (2013). CRISPR interference (CRISPRi) for sequence-specific control of gene expression. Nature Protocols, 8(11), 2180–2196. https://doi.org/10.1038/nprot.2013.132
  8. Farasat, I., Kushwaha, M., Collens, J., Easterbrook, M., Guido, M., & Salis, H. M. (2014). Efficient search, mapping, and optimization of multi‐protein genetic systems in diverse bacteria. Molecular systems biology, 10(6).
  9. Ng, C. Y., Farasat, I., Maranas, C. D., & Salis, H. M. (2015). Rational design of a synthetic Entner–Doudoroff pathway for improved and controllable NADPH regeneration. Metabolic engineering, 29, 86-96.