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It is a constitutive promoter from the surface (S)-layer protein (slp) promoter from <i>Lactobacillus acidophilus</i>, responsible for the high level of transcription of stable mRNAs coding the S-protein monomers (Boot & Pouwels, 1996; Boot et al., 1996). It was characterised in six strains of <i>Lactobacillus reuteri</i> and <i>Lactococcus lactis spp. cremoris</i> MG1363 by <a href="https://academic.oup.com/femsle/article/308/1/8/513999">Lizier et al, 2010.</a>
 
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P-slp is a constitutive promoter which can be used in <i>Lactobacillus reuteri</i> 10023C, and may have uses in other <i>Lactobacillus</i> species.
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The (S)-layer protein (slp) promoter is derived from <i>Lactobacillus acidophilus</i> and is responsible for the high level of transcription of stable mRNAs coding the S-protein monomers<sup>1,2</sup>. It was previously characterised in six strains of <i>Lactobacillus reuteri</i> and <i>Lactococcus lactis </i>spp.<i> cremoris</i> MG1363<sup>3</sup>. <br>
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The promoter shows high activity in <i>L. lactis </i>spp. <i>cremoris</i> MG1363, but low activity in <i>L. reuteri</i>.
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===Use by Team Oxford 2019===
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This part was used in our detection system to express AgrA2 and AgrC2 in <i>L. reuteri</i>. The composite part is: <partinfo>BBa_K3183102</partinfo>.
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===Characterisation===
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===References===
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1. Boot, H J et al. “The Lactobacillus acidophilus S-layer protein gene expression site comprises two consensus promoter sequences, one of which directs transcription of stable mRNA.” Journal of bacteriology vol. 178,18 (1996): 5388-94. doi:10.1128/jb.178.18.5388-5394.1996<br>
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2. Boot, Hein J., and Peter H. Pouwels. “Expression, Secretion and Antigenic Variation of Bacterial S-Layer Proteins.” Molecular Microbiology, vol. 21, no. 6, 1996, pp. 1117–1123., doi:10.1046/j.1365-2958.1996.711442.x.<br>
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3. Lizier, Michela, et al. “Comparison of Expression Vectors in Lactobacillus Reuteri Strains.” FEMS Microbiology Letters, vol. 308, no. 1, 2010, pp. 8–15., doi:10.1111/j.1574-6968.2010.01978.x.

Latest revision as of 21:16, 21 October 2019


Surface (S)-Layer Protein Constitutive Promoter for Lactobacillus sp.

P-slp is a constitutive promoter which can be used in Lactobacillus reuteri 10023C, and may have uses in other Lactobacillus species.

The (S)-layer protein (slp) promoter is derived from Lactobacillus acidophilus and is responsible for the high level of transcription of stable mRNAs coding the S-protein monomers1,2. It was previously characterised in six strains of Lactobacillus reuteri and Lactococcus lactis spp. cremoris MG13633.
The promoter shows high activity in L. lactis spp. cremoris MG1363, but low activity in L. reuteri.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Use by Team Oxford 2019

This part was used in our detection system to express AgrA2 and AgrC2 in L. reuteri. The composite part is: BBa_K3183102.


References

1. Boot, H J et al. “The Lactobacillus acidophilus S-layer protein gene expression site comprises two consensus promoter sequences, one of which directs transcription of stable mRNA.” Journal of bacteriology vol. 178,18 (1996): 5388-94. doi:10.1128/jb.178.18.5388-5394.1996
2. Boot, Hein J., and Peter H. Pouwels. “Expression, Secretion and Antigenic Variation of Bacterial S-Layer Proteins.” Molecular Microbiology, vol. 21, no. 6, 1996, pp. 1117–1123., doi:10.1046/j.1365-2958.1996.711442.x.
3. Lizier, Michela, et al. “Comparison of Expression Vectors in Lactobacillus Reuteri Strains.” FEMS Microbiology Letters, vol. 308, no. 1, 2010, pp. 8–15., doi:10.1111/j.1574-6968.2010.01978.x.