Difference between revisions of "Part:BBa K3208002"

 
 
(2 intermediate revisions by the same user not shown)
Line 3: Line 3:
 
<partinfo>BBa_K3208002 short</partinfo>
 
<partinfo>BBa_K3208002 short</partinfo>
  
This part is an expression module that produces Carcharin_1. Carcharin_1 is a putative antimicrobial peptide found in the genome of the great white shark.
 
  
Charcharin_1: SRSSRAGLQFPVGRVHRLQRK
+
This part is an expression module that produces Carcharin_2. Carcharin_2 is a putative antimicrobial peptide found in the genome of the great white shark.
 +
 
 +
 
 +
Charcharin_2: SRSSRAGLQLPAGRVHRLLRK
 +
 
  
 
The expression module contains:  
 
The expression module contains:  
Line 15: Line 18:
 
- start codon ATG (74-76)
 
- start codon ATG (74-76)
  
- Charcharin_1's CDS (77-139)
+
- Charcharin_2's CDS (77-139)
  
 
- stop codon (140-142)
 
- stop codon (140-142)
  
 
- T7 terminator (150-197).
 
- T7 terminator (150-197).
 +
 +
 +
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 21:15, 21 October 2019


Expression module that produces Carcharin_2


This part is an expression module that produces Carcharin_2. Carcharin_2 is a putative antimicrobial peptide found in the genome of the great white shark.


Charcharin_2: SRSSRAGLQLPAGRVHRLLRK


The expression module contains:

- T7 promoter (2-20)

- strong RBS (44-66)

- start codon ATG (74-76)

- Charcharin_2's CDS (77-139)

- stop codon (140-142)

- T7 terminator (150-197).



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 82
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 106
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 21