Difference between revisions of "Part:BBa K509005"
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<partinfo>BBa_K509005 short</partinfo> | <partinfo>BBa_K509005 short</partinfo> | ||
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+ | <h2>Usage and Biology</h2> | ||
+ | This is a Bioluminescent luciferase protein that emits green light, and so can be used as a reporter. It was isolated from the sea creature ''Gaussia princeps''. This version of the gene has been optimised for use in the algal species ''Chlamydomonas reinhardtii''. | ||
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+ | <h2>Improvement from SUSTech_Shenzhen 2019</h2> | ||
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+ | We designed <h4>[https://parts.igem.org/Part:BBa_K2986004 Biobrick BBa_K2086004, hGluc]</h4> as a improvement for the Gluc to the humanized Gaussia princeps which can be expressed in human cell lines. | ||
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Latest revision as of 21:08, 21 October 2019
GLuc
Usage and Biology
This is a Bioluminescent luciferase protein that emits green light, and so can be used as a reporter. It was isolated from the sea creature Gaussia princeps. This version of the gene has been optimised for use in the algal species Chlamydomonas reinhardtii.
Improvement from SUSTech_Shenzhen 2019
We designedBiobrick BBa_K2086004, hGluc
as a improvement for the Gluc to the humanized Gaussia princeps which can be expressed in human cell lines.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 205
Illegal NgoMIV site found at 550 - 1000COMPATIBLE WITH RFC[1000]