Difference between revisions of "Part:BBa K2984021"
 
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<partinfo>BBa_K2984021 parameters</partinfo>
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This part is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. To terminate the transcription of a gene, a signal on the DNA is required. The nucleotide sequence mediating this signal to the RNA-polymerase is called terminator. In 1986 a Chlamydomonas reinhardtii native gene for the small subunits of the ribulose bisphosphate carboxylase/oxygenase (Rubisco) was characterized (Goldschmidt-Clermont and Rahire, 1968; Stevens et al., 1996).
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This part is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. To terminate the transcription of a gene, a signal on the DNA is required. The nucleotide sequence mediating this signal to the RNA-polymerase is called terminator. In 1986 a <i>Chlamydomonas reinhardtii</i> native gene for the small subunits of the ribulose bisphosphate carboxylase/oxygenase (Rubisco) was characterized (Goldschmidt-Clermont and Rahire, 1968; Stevens et al., 1996).
 
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This part was designed to be used with the MoClo standard and has B6-C1 overhangs.
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K2984021 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2984021 SequenceAndFeatures</partinfo>
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==Characterization==
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<img src="https://static.igem.org/mediawiki/parts/a/ae/T--Humboldt_Berlin--RbcS2_B6-C1.png" alt="Cloning scheme L0-RbcS2 B6-C1" width="500">
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<figcaption>Fig.1 - Cloning scheme of L0-RbcS2_B6-C1 into <i>E. coli</i>. <B>A</B>  RbcS2 B6-C1 PCR product on agarose gel. <B>B + C</B> Successful transformation into <i>E. coli</i>. <B>D + E</B> Sequencing results of L0-RbcS2_B6-C1.</figcaption>
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===Functional Parameters===
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<partinfo>BBa_K2984009 parameters</partinfo>
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Goldschmidt-Clermont, M., & Rahire, M. (1986). Sequence, evolution and differential expression of the two genes encoding variant small subunits of ribulose bisphosphate carboxylase/oxygenase in Chlamydomonas reinhardtii. Journal of molecular biology, 191(3), 421-432.  
 
Goldschmidt-Clermont, M., & Rahire, M. (1986). Sequence, evolution and differential expression of the two genes encoding variant small subunits of ribulose bisphosphate carboxylase/oxygenase in Chlamydomonas reinhardtii. Journal of molecular biology, 191(3), 421-432.  
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<li>Stevens, D. R., Purton, S., & Rochaix, J.-D. (1996). The bacterial phleomycin resistance geneble as a dominant selectable marker inChlamydomonas. Molecular and General Genetics MGG, 251(1), 23–30. https://doi.org/10.1007/BF02174340
 
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Latest revision as of 21:04, 21 October 2019


RbcS2 - Terminator B6-C1

This part is a part of the Chlamy-HUB-Collection. To terminate the transcription of a gene, a signal on the DNA is required. The nucleotide sequence mediating this signal to the RNA-polymerase is called terminator. In 1986 a Chlamydomonas reinhardtii native gene for the small subunits of the ribulose bisphosphate carboxylase/oxygenase (Rubisco) was characterized (Goldschmidt-Clermont and Rahire, 1968; Stevens et al., 1996).

This part was designed to be used with the MoClo standard and has B6-C1 overhangs.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 255
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

Cloning scheme L0-RbcS2 B6-C1
Fig.1 - Cloning scheme of L0-RbcS2_B6-C1 into E. coli. A RbcS2 B6-C1 PCR product on agarose gel. B + C Successful transformation into E. coli. D + E Sequencing results of L0-RbcS2_B6-C1.


References

  1. Goldschmidt-Clermont, M., & Rahire, M. (1986). Sequence, evolution and differential expression of the two genes encoding variant small subunits of ribulose bisphosphate carboxylase/oxygenase in Chlamydomonas reinhardtii. Journal of molecular biology, 191(3), 421-432.
  2. Stevens, D. R., Purton, S., & Rochaix, J.-D. (1996). The bacterial phleomycin resistance geneble as a dominant selectable marker inChlamydomonas. Molecular and General Genetics MGG, 251(1), 23–30. https://doi.org/10.1007/BF02174340