Difference between revisions of "Part:BBa K3110012"
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<partinfo>BBa_K3110012 short</partinfo> | <partinfo>BBa_K3110012 short</partinfo> | ||
| − | L-Lactate Dehydrogenase (lldD) under the control of a weak promoter and a | + | This construct has L-Lactate Dehydrogenase (lldD) under the control of a weak promoter and a weak RBS. |
| + | lldD cleaves L-lactate to pyruvate. Our intention of using this part is to have control over the detection threshold of | ||
| + | L-Lactate by the lldPRD regulatory region. We have also designed other parts which produce various higher concentrations of lldD by varying the strength of the promoter and RBS controlling its production. | ||
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<partinfo>BBa_K3110012 parameters</partinfo> | <partinfo>BBa_K3110012 parameters</partinfo> | ||
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| + | <h1>Characterization </h1> | ||
| + | Due to shortage of time we could not characterize this part. | ||
Latest revision as of 21:02, 21 October 2019
Weak Promoter Weak RBS lldD
This construct has L-Lactate Dehydrogenase (lldD) under the control of a weak promoter and a weak RBS. lldD cleaves L-lactate to pyruvate. Our intention of using this part is to have control over the detection threshold of L-Lactate by the lldPRD regulatory region. We have also designed other parts which produce various higher concentrations of lldD by varying the strength of the promoter and RBS controlling its production.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1201
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 638
Characterization
Due to shortage of time we could not characterize this part.