Difference between revisions of "Part:BBa K3042000"

Revision as of 20:56, 21 October 2019

Dinoflagellate Codon Optimized RFP

This part is a codon-optimized version of RFP for use in use in Dinoflagellates, a single-celled organism. This part was created to be used in DinoIII, a dinoflagellate backbone vector. The sequence for this part was synthesized via Snapgene. Considering dinoflagellates have many codons that code for the same amino acids, favorable codons of the dinoflagellates were chosen using the chart below to ensure the translation of the red fluorescent protein. This part can be used as a reporter or indicator of a successful transformation. Positive results are demonstrated by red fluorescence emitted from the cells.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 13
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 696
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 13
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 13
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3042000 short</partinfo>
-This is a red fluorescent protein coding sequence that has been codon optimized for use in dinoflagellates.
+This part is a codon-optimized version of RFP for use in use in Dinoflagellates, a single-celled organism. This part was created to be used in DinoIII, a dinoflagellate backbone vector.  The sequence for this part was synthesized via Snapgene. Considering dinoflagellates have many codons that code for the same amino acids, favorable codons of the dinoflagellates were chosen using the chart below to ensure the translation of the red fluorescent protein. This part can be used as a reporter or indicator of a successful transformation. Positive results are demonstrated by red fluorescence emitted from the cells.
 <!-- Add more about the biology of this part here