Difference between revisions of "Part:BBa K3304000"
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<partinfo>BBa_K3304000 short</partinfo> | <partinfo>BBa_K3304000 short</partinfo> | ||
| − | This is the Input Gate for our Chemical Reaction Network. The oligo input is DTG | + | This is the Input Gate for our Chemical Reaction Network. The oligo input is DTG [[Part:BBa_K3304005]] with initiates the strand displacement cascades. Other auxiliary strands for this gate are BTD [[Part:BBa_K3304006]] and NTB [[Part:BBa_K3304007]] who conclude the reactions and facilitate their trajectory to the desired direction. The output of this gate is TBB [[Part:BBa_K3304006]] which constitutes the input for the downstream Join Gate [[Part:BBa_K3304001]]. The part comes with two PvuII cut sites and three Nt.BstNBI nicking sites that facilitate the extraction of the gate from plasmid DNA and prepare the double-stranded block for strand displacement reactions. |
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Revision as of 20:46, 21 October 2019
Input Gate with NF-κB p65 consensus sequence
This is the Input Gate for our Chemical Reaction Network. The oligo input is DTG Part:BBa_K3304005 with initiates the strand displacement cascades. Other auxiliary strands for this gate are BTD Part:BBa_K3304006 and NTB Part:BBa_K3304007 who conclude the reactions and facilitate their trajectory to the desired direction. The output of this gate is TBB Part:BBa_K3304006 which constitutes the input for the downstream Join Gate Part:BBa_K3304001. The part comes with two PvuII cut sites and three Nt.BstNBI nicking sites that facilitate the extraction of the gate from plasmid DNA and prepare the double-stranded block for strand displacement reactions.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]