Difference between revisions of "Part:BBa K3137004"

Latest revision as of 20:34, 21 October 2019

mCherry

The mCherry can express red fluorescent protein and it is used as a reporter gene in the process of reporting the number of cells.

Usage and Biology

When phages infect bacteria into the burst period (about 20 min), the inducible promoter PglcF(BBa_K3137003) will induce expression of the red fluorescent protein gene mCherry, which will make the bacteria display red fluorescence.

Characterization of PglcF

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 352
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 352
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 352
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 352
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3137004 short</partinfo>
-The mCherry can express red fluorescent protein and it is used as a marker gene in the process of reporting the number of cells.
+The mCherry can express red fluorescent protein and it is used as a reporter gene in the process of reporting the number of cells.
 ===Usage and Biology===
-When phages infect bacteria into the burst period (about 20 min), the inducible promoter PglcF will induce expression of the red fluorescent protein gene mCherry([https://parts.igem.org/Part:BBa_K3137004# BBa_K3137004]), which will make the bacteria display red fluorescence.
+When phages infect bacteria into the burst period (about 20 min), the inducible promoter PglcF([https://parts.igem.org/Part:BBa_K3137003# BBa_K3137003]) will induce expression of the red fluorescent protein gene mCherry, which will make the bacteria display red fluorescence.
 [[Image:PglcF.png|500px|thumb|center|Characterization of PglcF]]