Difference between revisions of "Part:BBa K2992033"

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===Characterisation===
 
===Characterisation===
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This part is the ribosome binding site for the translation of the <i> adc </i> gene from <i> C. acetobutylicum </i>. This was integrated in to the larger composite parts:[https://parts.igem.org/Part:BBa_K2992028 BBa_K2992028], [https://parts.igem.org/Part:BBa_K2992029 BBa_K2992029], [https://parts.igem.org/Part:BBa_K2992030 BBa_K2992030], [https://parts.igem.org/Part:BBa_K2992034 BBa_K2992034], [https://parts.igem.org/Part:BBa_K2992035 BBa_K2992035] and [https://parts.igem.org/Part:BBa_K2992036 BBa_K2992036]. See [https://2019.igem.org/Team:Nottingham/Results results page]for more  
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This part is the ribosome binding site for the translation of the <i> adc </i> gene from <i> C. acetobutylicum </i>. This was integrated in to the larger composite parts:         [https://parts.igem.org/Part:BBa_K2992028 BBa_K2992028], [https://parts.igem.org/Part:BBa_K2992029 BBa_K2992029], [https://parts.igem.org/Part:BBa_K2992030 BBa_K2992030], [https://parts.igem.org/Part:BBa_K2992034 BBa_K2992034], [https://parts.igem.org/Part:BBa_K2992035 BBa_K2992035] and [https://parts.igem.org/Part:BBa_K2992036 BBa_K2992036]. See [https://2019.igem.org/Team:Nottingham/Results results page]for more  
  
  

Revision as of 20:30, 21 October 2019


RBS region for the adc gene from C. acetobutylicum.

RBS region for the adc gene from C. acetobutylicum.

Usage and Biology

This parts entry represents the RBS for the acetoacetate decarboxylase adc gene in C. acetobutylicum. The gene product of adc catalyses the decarboxylation of acetoacetate into acetone and CO2. adc is a central component of our acetone production pathway to validate the use of volatile reporters to predict botulinum toxin production following food manufacturing processes.


Characterisation

This part is the ribosome binding site for the translation of the adc gene from C. acetobutylicum . This was integrated in to the larger composite parts: BBa_K2992028, BBa_K2992029, BBa_K2992030, BBa_K2992034, BBa_K2992035 and BBa_K2992036. See results pagefor more


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Unknown
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    INCOMPATIBLE WITH RFC[23]
    Unknown
  • 25
    INCOMPATIBLE WITH RFC[25]
    Unknown
  • 1000
    COMPATIBLE WITH RFC[1000]

References

Cornillot, E., Croux, C. and Soucaille, P. (1997). Physical and genetic map of the Clostridium acetobutylicum ATCC 824 chromosome. Journal of Bacteriology, 179(23), pp.7426-7434.