Difference between revisions of "Part:BBa K3110048:Design"

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===Design Notes===
 
===Design Notes===
  
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Codon optimisation was done using Twist Codon optimisation too to make it synthesis compatible in E.coli
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Codon optimisation was done using Twist Codon optimisation too to make it synthesis compatible in E.coli. The FLAG-tag at the end can be used to detect IL12 produced by E.coli.
 +
Also took help from the sequence designed by UNICAMP EMSE Brazil team (iGEM2011) - [[Part:BBa_K554005]]
  
 
===Source===
 
===Source===
  
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fasdfas
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The whole sequence was synthesized by Twist Bioscience.
  
 
===References===
 
===References===
 +
<ul>
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<li>K. R. Morris et al, Expression of biologically active recombinant porcine interleukin-12 from Escherichia coli, Veterinary Immunology and Immunopathology, 2008, 126 Link to PubMed</li>
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<li>Sperandio V, Torres AG, Kaper JB (2002) Quorum sensing Escherichia coli regulators B and C (QseBC): a novel two-component regulatory system involved in the regulation of flagella and motility by quorum sensing in E. coli. Mol Microbiol 43: 809–821</li>
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<li>[[Part:BBa_K554005]] designed by UNICAMP EMSE Brazil team</li>
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</ul>

Latest revision as of 20:12, 21 October 2019


IL12 FLAG-tag (Codon Optimized)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimisation was done using Twist Codon optimisation too to make it synthesis compatible in E.coli. The FLAG-tag at the end can be used to detect IL12 produced by E.coli. Also took help from the sequence designed by UNICAMP EMSE Brazil team (iGEM2011) - Part:BBa_K554005

Source

The whole sequence was synthesized by Twist Bioscience.

References

  • K. R. Morris et al, Expression of biologically active recombinant porcine interleukin-12 from Escherichia coli, Veterinary Immunology and Immunopathology, 2008, 126 Link to PubMed
  • Sperandio V, Torres AG, Kaper JB (2002) Quorum sensing Escherichia coli regulators B and C (QseBC): a novel two-component regulatory system involved in the regulation of flagella and motility by quorum sensing in E. coli. Mol Microbiol 43: 809–821
  • Part:BBa_K554005 designed by UNICAMP EMSE Brazil team