Difference between revisions of "Part:BBa K2908681"

Latest revision as of 20:11, 21 October 2019

s(ESR1)p-miR101spe-EGFP

The CSU_CHINA iGEM team 2019 designed a fusion protein consisting of miR101 sponge and EGFP. Therefore, we used our s(ESR1)p(BBa_K2908111) as a specific promoter.At the same time we used our miR101-sponge(BBa_K2908669) to bind different concentrations of miR101 and fuse it to 3' end so that can regulate the transactivation of EGFP which is a marker.In this way we can ensure the specificity and independence of this protein.

Physical map of s(ESR1)p-miR101spe-EGFP

The construction method is the same as module1 (BBa_2908671), only except the paired sequence of each inserted fragment, which shows in the following picture.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 155
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 200
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 The CSU_CHINA iGEM team 2019 designed a fusion protein consisting of miR101 sponge and EGFP. Therefore, we used our s(ESR1)p(BBa_K2908111) as a specific promoter.At the same time we used our miR101-sponge(BBa_K2908669) to bind different concentrations of miR101 and fuse it to 3' end so that can regulate the transactivation of EGFP which is a marker.In this way we can ensure the specificity and independence of this protein.
-https://static.igem.org/mediawiki/parts/3/3a/T--CSU_CHINA--module2.png
+<center>https://2019.igem.org/wiki/images/a/a5/T--CSU_CHINA--2908681.jpg</center><br/>
+<center>Physical map of s(ESR1)p-miR101spe-EGFP</center><br/>
+The construction method is the same as module1 (BBa_2908671), only except the paired sequence of each inserted fragment, which shows in the following picture.<br/>
 <!-- Add more about the biology of this part here
 ===Usage and Biology===