Difference between revisions of "Part:BBa K3015011"
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===Usage and Biology=== | ===Usage and Biology=== | ||
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This is the genetic device we realized in the lab with a transcriptional Theophylline-Riboswitch. T7-Polymerase will be expressed in the Presence of Theophylline, which will further induce the strong expression of amilCP, a blue chromoprotein, that acts as visual readout. The designed and constructed system can easily be adapted for detection of various disease-causing agents by just changing the riboswitch-sequence according to the target. | This is the genetic device we realized in the lab with a transcriptional Theophylline-Riboswitch. T7-Polymerase will be expressed in the Presence of Theophylline, which will further induce the strong expression of amilCP, a blue chromoprotein, that acts as visual readout. The designed and constructed system can easily be adapted for detection of various disease-causing agents by just changing the riboswitch-sequence according to the target. | ||
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Revision as of 20:09, 21 October 2019
Riboswitch Sensor cassette
This genetic device was used to proof the functionality of a Riboswitch-based diagnostic method.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 2886
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2820
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 2886
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 2886
- 1000COMPATIBLE WITH RFC[1000]