Difference between revisions of "Part:BBa K3280005"

Latest revision as of 20:04, 21 October 2019

Terminator tpr + MerR+ bidirectional Promotor + RBS (Mer operon) segment of BBa_K1355001, a biobrick designed to be the central piece of different genetic constructions related to mercury, which is composed by a bidirectional promoter, a operon expression regulator (MerR), a carrier protein (MerP) and a mercury transport protein (MerT). BBa_k3280005 has only a terminator, a RBS, MerR domain and a bidirectional promoter that has a dual function: in reverse, transcription of the MerR regulator protein and in forward, it is opened for insertion.

In the absence of mercury MerR represses transcription by binding tightly to the mer operator region; when mercury is present the dimeric complex binds a single ion and becomes a potent transcriptional activator, while remaining bound to the mer site.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_K3280005 short</partinfo>
+segment of BBa_K1355001, a biobrick designed to be the central piece of different genetic constructions related to mercury, which is composed by a bidirectional promoter, a operon expression regulator (MerR), a carrier protein (MerP) and a mercury transport protein (MerT).
-This part is a segment of BBa_K1355001. This biobrick has a bidirectional promoter, having dual functions: A) In reverse: transcription of the MerR regulator protein; and B) In forward: opened for insertion.
+	BBa_k3280005 has only a terminator, a RBS, MerR domain and a bidirectional promoter that has a dual function: in reverse, transcription of the MerR regulator protein and in forward, it is opened for insertion.
+ In the absence of mercury MerR represses transcription by binding tightly to the mer operator region; when mercury is present the dimeric complex binds a single ion and becomes a potent transcriptional activator, while remaining bound to the mer site.
 <!-- Add more about the biology of this part here