Difference between revisions of "Part:BBa K3286001"
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<partinfo>BBa_K3286001 short</partinfo> | <partinfo>BBa_K3286001 short</partinfo> | ||
| − | Non-targeting Spacer and single guide (sg)RNA scaffold of <i>Streptococcus pyogenes</i> (Spy)Cas9 used and further characterised in the composite [[Part:BBa_K3286003]]. | + | Non-targeting Spacer and single guide (sg)RNA scaffold of <i>Streptococcus pyogenes</i> (Spy)Cas9 used and further characterised in the composite [[Part:BBa_K3286003]]. |
| + | The sgRNA is a chimeric non-coding RNA mediating Cas9 activation and target binding and mainly consists of three parts: a 20 nt long spacer binding target DNA, a 42 nt Cas9 binding hairpin and a 40 nt terminator [1]. | ||
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Latest revision as of 19:59, 21 October 2019
single guide RNA Cas9
Non-targeting Spacer and single guide (sg)RNA scaffold of Streptococcus pyogenes (Spy)Cas9 used and further characterised in the composite Part:BBa_K3286003. The sgRNA is a chimeric non-coding RNA mediating Cas9 activation and target binding and mainly consists of three parts: a 20 nt long spacer binding target DNA, a 42 nt Cas9 binding hairpin and a 40 nt terminator [1].
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]