Difference between revisions of "Part:BBa K3286001"

 
 
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<partinfo>BBa_K3286001 short</partinfo>
  
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Non-targeting Spacer and single guide (sg)RNA scaffold of <i>Streptococcus pyogenes</i> (Spy)Cas9 used and further characterised in the composite [[Part:BBa_K3286003]].
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The sgRNA is a chimeric non-coding RNA mediating Cas9 activation and target binding and mainly consists of three parts: a 20 nt long spacer binding target DNA, a 42 nt Cas9 binding hairpin and a 40 nt terminator [1].
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3286001 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K3286001 parameters</partinfo>
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Latest revision as of 19:59, 21 October 2019

single guide RNA Cas9

Non-targeting Spacer and single guide (sg)RNA scaffold of Streptococcus pyogenes (Spy)Cas9 used and further characterised in the composite Part:BBa_K3286003. The sgRNA is a chimeric non-coding RNA mediating Cas9 activation and target binding and mainly consists of three parts: a 20 nt long spacer binding target DNA, a 42 nt Cas9 binding hairpin and a 40 nt terminator [1].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]