Difference between revisions of "Part:BBa K3156888"
Marshalliu (Talk | contribs) |
Marshalliu (Talk | contribs) |
||
| Line 12: | Line 12: | ||
</figcaption> | </figcaption> | ||
</figure> | </figure> | ||
| − | + | <p> 111111111111111111111111111111111111111111111111111111111111111111111111111</p> | |
<figure> | <figure> | ||
<p style="text-align:center;"><img src="https://2019.igem.org/wiki/images/0/06/T--SHSBNU_China---dual_repair.png" width = "700" height ="500"/> | <p style="text-align:center;"><img src="https://2019.igem.org/wiki/images/0/06/T--SHSBNU_China---dual_repair.png" width = "700" height ="500"/> | ||
Revision as of 19:53, 21 October 2019
pLac Promoter-ssDNA[sfGFP(ON)]-Ec86-Beta
We combined msd-msr cassette, Reverse Transcriptase Ec-86 and Beta recombinase together to create BBa_K3156888. Msd-msr cassette can produce mRNA for reverse transcription then
Figure 1.Circuit design of BBa_K3156888(sfgfp reverse).
111111111111111111111111111111111111111111111111111111111111111111111111111
Figure 1.Repairing result of BBa_K3156888(sfgfp reverse).
Usage and Biology
Reference [1]F. Farzadfard, T. K. Lu, Science 346,1256272 (2014). DOI: 10.1126/science.1256272 Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 9
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1
Illegal XhoI site found at 516 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]