Difference between revisions of "Part:BBa K3051001"
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− | <div style="width:50%">Candida Antartica Lipase A (CALA) is a lipase extracted from the yeast Candida Antartica. It has been shown to work at very low temperatures. CALA has been shown to be very receptive to C8 p nitrophenol esters, compared to C4 and >C12 esters. | + | <div style="width:50%;float:left">Candida Antartica Lipase A (CALA) is a lipase extracted from the yeast Candida Antartica. It has been shown to work at very low temperatures. CALA has been shown to be very receptive to C8 p nitrophenol esters, compared to C4 and >C12 esters. |
It has also been shown to be very sensitive to phenylmethylsulfonyl fluoride and high alcohol contents, causing a decrease in activity of as low as 50% compared to its control.[1] | It has also been shown to be very sensitive to phenylmethylsulfonyl fluoride and high alcohol contents, causing a decrease in activity of as low as 50% compared to its control.[1] |
Revision as of 19:47, 21 October 2019
Candida Antarctica Lipase A
Candida Antartica Lipase A (CALA) is a lipase extracted from the yeast Candida Antartica. It has been shown to work at very low temperatures. CALA has been shown to be very receptive to C8 p nitrophenol esters, compared to C4 and >C12 esters.
It has also been shown to be very sensitive to phenylmethylsulfonyl fluoride and high alcohol contents, causing a decrease in activity of as low as 50% compared to its control.[1]
It has a kDa of 49.3 and has been shown to react with p nitrophenol octanoate, to give p nitrophenol. Its molar extinction coefficient in water has been calculated to be 54570 M-1cm-1 (using ProtoPram ExPaSy tool). Its 3D structure has been calculated to be as follows:
Sources
1. Lan, Dong-Ming et al. “A novel cold-active lipase from Candida albicans: cloning, expression and characterization of the recombinant enzyme.” International journal of molecular sciences vol. 12,6 (2011): 3950-65. doi:10.3390/ijms12063950
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]