Difference between revisions of "Part:BBa K3286000:Design"

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===Design Notes===
 
===Design Notes===
 
non-codon optimised
 
non-codon optimised
−
 
−
 
  
 
===Source===
 
===Source===
  
−
ordered via IDT
+
retrieved from NCBI
  
 
===References===
 
===References===
 
Rauch, B. J., Silvis, M. R., Hultquist, J. F., Waters, C. S., McGregor, M. J., Krogan, N. J., & Bondy-Denomy, J. (2017). Inhibition of CRISPR-Cas9 with bacteriophage proteins. Cell, 168(1-2), 150-158.
 
Rauch, B. J., Silvis, M. R., Hultquist, J. F., Waters, C. S., McGregor, M. J., Krogan, N. J., & Bondy-Denomy, J. (2017). Inhibition of CRISPR-Cas9 with bacteriophage proteins. Cell, 168(1-2), 150-158.
 +
 +
Kim, I., Jeong, M., Ka, D., Han, M., Kim, N. K., Bae, E., & Suh, J. Y. (2018). Solution structure and dynamics of anti-CRISPR AcrIIA4, the Cas9 inhibitor. Scientific reports, 8(1), 3883.
 +
 +
Dong, D., Guo, M., Wang, S., Zhu, Y., Wang, S., Xiong, Z., ... & Huang, Z. (2017). Structural basis of CRISPR–SpyCas9 inhibition by an anti-CRISPR protein. Nature, 546(7658), 436.
 +
 +
Yang, H., & Patel, D. J. (2017). Inhibition mechanism of an anti-CRISPR suppressor AcrIIA4 targeting SpyCas9. Molecular cell, 67(1), 117-127.
 +
 +
Shin, J., Jiang, F., Liu, J. J., Bray, N. L., Rauch, B. J., Baik, S. H., ... & Doudna, J. A. (2017). Disabling Cas9 by an anti-CRISPR DNA mimic. Science advances, 3(7), e1701620.
 +
 +
Trasanidou, D., Gerós, A. S., Mohanraju, P., Nieuwenweg, A. C., Nobrega, F. L., & Staals, R. H. (2019). Keeping crispr in check: diverse mechanisms of phage-encoded anti-crisprs. FEMS microbiology letters, 366(9), fnz098.

Latest revision as of 19:47, 21 October 2019


AcrIIA4


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

non-codon optimised

Source

retrieved from NCBI

References

Rauch, B. J., Silvis, M. R., Hultquist, J. F., Waters, C. S., McGregor, M. J., Krogan, N. J., & Bondy-Denomy, J. (2017). Inhibition of CRISPR-Cas9 with bacteriophage proteins. Cell, 168(1-2), 150-158.

Kim, I., Jeong, M., Ka, D., Han, M., Kim, N. K., Bae, E., & Suh, J. Y. (2018). Solution structure and dynamics of anti-CRISPR AcrIIA4, the Cas9 inhibitor. Scientific reports, 8(1), 3883.

Dong, D., Guo, M., Wang, S., Zhu, Y., Wang, S., Xiong, Z., ... & Huang, Z. (2017). Structural basis of CRISPR–SpyCas9 inhibition by an anti-CRISPR protein. Nature, 546(7658), 436.

Yang, H., & Patel, D. J. (2017). Inhibition mechanism of an anti-CRISPR suppressor AcrIIA4 targeting SpyCas9. Molecular cell, 67(1), 117-127.

Shin, J., Jiang, F., Liu, J. J., Bray, N. L., Rauch, B. J., Baik, S. H., ... & Doudna, J. A. (2017). Disabling Cas9 by an anti-CRISPR DNA mimic. Science advances, 3(7), e1701620.

Trasanidou, D., Gerós, A. S., Mohanraju, P., Nieuwenweg, A. C., Nobrega, F. L., & Staals, R. H. (2019). Keeping crispr in check: diverse mechanisms of phage-encoded anti-crisprs. FEMS microbiology letters, 366(9), fnz098.