Difference between revisions of "Part:BBa K3002228"

Revision as of 19:28, 21 October 2019

L2 spectinomycin resistance + L1_connector_Pos2 + ccA_MHETase_SP20His

This composite part contains a spectinomycin resistance (BBa_K3002102) and the MHETase with the secretion signal cCA (BBa_K3002123) fused with an SP20His-tag for easy purification via Ni-NTA column and enhanced secretion. Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 3738
Illegal PstI site found at 4062
Illegal PstI site found at 4405
Illegal PstI site found at 5215
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 2660
Illegal PstI site found at 3738
Illegal PstI site found at 4062
Illegal PstI site found at 4405
Illegal PstI site found at 5215
Illegal NotI site found at 4073
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 4983
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 3738
Illegal PstI site found at 4062
Illegal PstI site found at 4405
Illegal PstI site found at 5215
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 3738
Illegal PstI site found at 4062
Illegal PstI site found at 4405
Illegal PstI site found at 5215
Illegal NgoMIV site found at 1401
Illegal NgoMIV site found at 1584
Illegal NgoMIV site found at 1694
Illegal NgoMIV site found at 3671
Illegal NgoMIV site found at 4132
Illegal NgoMIV site found at 4162
Illegal NgoMIV site found at 4477
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3002228 short</partinfo>
-This composite part contains a hygromycin resistance (BBa_K3002119) and the mutant PETase with the cCA (BBa_K3002109) fused with an SP20HA-tag for easy detection via HA-antibody and enhanced secretion.
+This composite part contains a spectinomycin resistance (BBa_K3002102) and the MHETase with the secretion signal cCA (BBa_K3002123) fused with an SP20His-tag for easy purification via Ni-NTA column and enhanced secretion.
 <!-- Add more about the biology of this part here
 ===Usage and Biology===