Difference between revisions of "Part:BBa K3081108"
 
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<partinfo>BBa_K3081108 short</partinfo>
 
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The 20 bp sgRNA targets to the R1+ DnaA box on E.coli genome replication initiation region, OriC. In natural situations, R1+ is a high affinity box for DnaA binding. By blocking the binding of DnaA protein to R1+ box, severe arrest and inhibition of genome replication initiation is achieved. For more detailed information, see <partinfo>BBa_K3081058</partinfo>
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The 20 bp sgRNA targets to the R1+ DnaA box on <i>E.coli</i> genome replication initiation region, OriC. In natural situations, R1+ is a high affinity box for DnaA binding. By blocking the binding of DnaA protein to R1+ box, severe arrest and inhibition of genome replication initiation is achieved. For more detailed information, see <partinfo>BBa_K3081058</partinfo>
  
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<center>
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https://2019.igem.org/wiki/images/0/0a/T--Peking--box.png
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</center>
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<center>
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Figure1: Different boxes related to <i>E.coli</i> genome replication.This figure is adapted from the review of Costa, A. et al[1]
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</center>
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Reference:
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[1]Mott, M. L. , & Berger, J. M. . (2007). Dna replication initiation: mechanisms and regulation in bacteria. Nature Reviews Microbiology, 5(5), 343-354.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 19:18, 21 October 2019


a 20bp sequence targeting the R1 (bottom strand) DnaA box on E.coli oriC

The 20 bp sgRNA targets to the R1+ DnaA box on E.coli genome replication initiation region, OriC. In natural situations, R1+ is a high affinity box for DnaA binding. By blocking the binding of DnaA protein to R1+ box, severe arrest and inhibition of genome replication initiation is achieved. For more detailed information, see BBa_K3081058


T--Peking--box.png

Figure1: Different boxes related to E.coli genome replication.This figure is adapted from the review of Costa, A. et al[1]


Reference: [1]Mott, M. L. , & Berger, J. M. . (2007). Dna replication initiation: mechanisms and regulation in bacteria. Nature Reviews Microbiology, 5(5), 343-354.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]