Difference between revisions of "Part:BBa K3081063"

Latest revision as of 19:13, 21 October 2019

pBAD-dCas9-J23119-pL05

"This composite part is the principal design of the inducible CRISPR-based DNA replication interference system, with the 20bp sequence targeting the IHF binding region of plasmid replication origin pSC101,using in the CRISPRri systemBBa_K3081007 to control the plasmid copy number."

Reference：

[1]Wolański M, Donczew R, Zawilakpawlik A, et al. oriC-encoded instructions for the initiation of bacterial chromosome replication.[J]. Frontiers in Microbiology, 2015, 5(735):735.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1205
Illegal NheI site found at 5459
Illegal NheI site found at 5482
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1470
Illegal BamHI site found at 1144
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 979
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 961

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3081063 short</partinfo>
-"This composite part is the principal design of the inducible CRISPR-based DNA
+"This composite part is the principal design of the inducible CRISPR-based DNA replication interference system, with the 20bp sequence targeting the IHF binding region of plasmid replication origin pSC101,using in the CRISPRri system<partinfo>BBa_K3081007</partinfo> to control the  plasmid copy number."
-replication interference system, with the 20bp sequence targeting the IHF binding region
-of plasmid replication origin pSC101,using in the CRISPRri system<partinfo>BBa_K3081007</partinfo> to control the  plasmid copy number."
+<center>
+https://2019.igem.org/wiki/images/c/cf/T--Peking--plasmid_copy_number_pL01.png
+</center>
+Reference：
+[1]Wolański M, Donczew R, Zawilakpawlik A, et al. oriC-encoded instructions for the initiation of bacterial chromosome replication.[J]. Frontiers in Microbiology, 2015, 5(735):735.