Difference between revisions of "Part:BBa K3081015"
 
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<partinfo>BBa_K3081015 short</partinfo>
 
<partinfo>BBa_K3081015 short</partinfo>
  
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This composite part is the principal design of the inducible CRISPR-based DNA replication interference system, with the 20 bp sgRNA targeting to the R3+ DnaA box on E.coli genome replication initiation region, OriC. In natural situations, R3 is a low affinity box for DnaA binding. By blocking the binding of DnaA protein to R3 box, moderate arrest and inhibition of genome replication initiation is achieved.
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This composite part is the principal design of the inducible CRISPR-based DNA replication interference system, with the 20 bp sgRNA targeting to the R3+ DnaA box on <i>E.coli</i> genome replication initiation region, OriC. In natural situations, R3 is a low affinity box for DnaA binding. By blocking the binding of DnaA protein to R3 box, moderate arrest and inhibition of genome replication initiation is achieved.
  
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<H1>Experiment</H1>
 
For more detailed information, see <partinfo>BBa_K3081058</partinfo>
 
For more detailed information, see <partinfo>BBa_K3081058</partinfo>
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https://2019.igem.org/wiki/images/f/fc/T--Peking--logo_results.png
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Reference:
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[1]Wolański M, Donczew R, Zawilakpawlik A, et al. oriC-encoded instructions for the initiation of bacterial chromosome replication.[J]. Frontiers in Microbiology, 2015, 5(735):735.  
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 19:09, 21 October 2019


pBAD-dCas9-J23119-R3+

This composite part is the principal design of the inducible CRISPR-based DNA replication interference system, with the 20 bp sgRNA targeting to the R3+ DnaA box on E.coli genome replication initiation region, OriC. In natural situations, R3 is a low affinity box for DnaA binding. By blocking the binding of DnaA protein to R3 box, moderate arrest and inhibition of genome replication initiation is achieved.

Experiment

For more detailed information, see BBa_K3081058


Reference:

[1]Wolański M, Donczew R, Zawilakpawlik A, et al. oriC-encoded instructions for the initiation of bacterial chromosome replication.[J]. Frontiers in Microbiology, 2015, 5(735):735.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
    Illegal NheI site found at 5459
    Illegal NheI site found at 5482
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1470
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961